Evidence map›Paper›PMID 26553649›Full record

ArticlePlant physiology2016

Regulation of Primary Metabolism in Response to Low Oxygen Availability as Revealed by Carbon and Nitrogen Isotope Redistribution.

Carla António, Carola Päpke, Marcio Rocha, Houssein Diab, Anis M Limami, Toshihiro Obata, Alisdair R Fernie, Joost T van Dongen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.

0numbers the graph read from it
0cells of the map it votes in
58citing papers in PubMed
20.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

58 citing papers in PubMed, 134 citations in OpenAlex.

  1. Ribosome stalling position, spacing, and A-site occupancy impact translation and cotranslational mRNA decay in plants.Proceedings of the National Academy of Sciences of the United States of America · 2026
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  11. Truncation of the calmodulin binding domain in rice glutamate decarboxylase 4 (Molecular breeding : new strategies in plant improvement · 2024
    Article
  12. Article
  13. Plant Life with and without Oxygen: A Metabolomics Approach.International journal of molecular sciences · 2023
    Review
  14. Review
  15. Article
  16. Review
  17. Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  18. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 4 countries.

Carla AntónioEnergy Metabolism Group (C.A., C.P., M.R., J.T.v.D.) and Central Metabolism Group (T.O., A.R.F.), Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany;Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier-Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal (C.A.);Departamento de Produção Animal e Vegetal, Faculdade de Ciências Agrárias, Universidade Federal do Amazonas, Manaus, Amazonas 69082-653, Brazil (M.R.);University of Angers (H.D., A.M.L.) and Institut National de la Recherche Agronomique (A.M.L.), Unité Mixte de Recherche 1345 IRHS, SFR 4207 QUASAV, F-49045 Angers, France; andInstitute for Biology I, RWTH Aachen University, D-52056 Aachen, Germany (J.T.v.D.) antonio@itqb.unl.pt.ORCID http://orcid.org/0000-0001-6747-1243
Carola PäpkeEnergy Metabolism Group (C.A., C.P., M.R., J.T.v.D.) and Central Metabolism Group (T.O., A.R.F.), Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany;Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier-Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal (C.A.);Departamento de Produção Animal e Vegetal, Faculdade de Ciências Agrárias, Universidade Federal do Amazonas, Manaus, Amazonas 69082-653, Brazil (M.R.);University of Angers (H.D., A.M.L.) and Institut National de la Recherche Agronomique (A.M.L.), Unité Mixte de Recherche 1345 IRHS, SFR 4207 QUASAV, F-49045 Angers, France; andInstitute for Biology I, RWTH Aachen University, D-52056 Aachen, Germany (J.T.v.D.).
Marcio RochaEnergy Metabolism Group (C.A., C.P., M.R., J.T.v.D.) and Central Metabolism Group (T.O., A.R.F.), Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany;Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier-Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal (C.A.);Departamento de Produção Animal e Vegetal, Faculdade de Ciências Agrárias, Universidade Federal do Amazonas, Manaus, Amazonas 69082-653, Brazil (M.R.);University of Angers (H.D., A.M.L.) and Institut National de la Recherche Agronomique (A.M.L.), Unité Mixte de Recherche 1345 IRHS, SFR 4207 QUASAV, F-49045 Angers, France; andInstitute for Biology I, RWTH Aachen University, D-52056 Aachen, Germany (J.T.v.D.).ORCID http://orcid.org/0000-0003-4937-3855
Houssein DiabEnergy Metabolism Group (C.A., C.P., M.R., J.T.v.D.) and Central Metabolism Group (T.O., A.R.F.), Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany;Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier-Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal (C.A.);Departamento de Produção Animal e Vegetal, Faculdade de Ciências Agrárias, Universidade Federal do Amazonas, Manaus, Amazonas 69082-653, Brazil (M.R.);University of Angers (H.D., A.M.L.) and Institut National de la Recherche Agronomique (A.M.L.), Unité Mixte de Recherche 1345 IRHS, SFR 4207 QUASAV, F-49045 Angers, France; andInstitute for Biology I, RWTH Aachen University, D-52056 Aachen, Germany (J.T.v.D.).
Anis M LimamiEnergy Metabolism Group (C.A., C.P., M.R., J.T.v.D.) and Central Metabolism Group (T.O., A.R.F.), Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany;Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier-Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal (C.A.);Departamento de Produção Animal e Vegetal, Faculdade de Ciências Agrárias, Universidade Federal do Amazonas, Manaus, Amazonas 69082-653, Brazil (M.R.);University of Angers (H.D., A.M.L.) and Institut National de la Recherche Agronomique (A.M.L.), Unité Mixte de Recherche 1345 IRHS, SFR 4207 QUASAV, F-49045 Angers, France; andInstitute for Biology I, RWTH Aachen University, D-52056 Aachen, Germany (J.T.v.D.).ORCID http://orcid.org/0000-0002-9985-2363
Toshihiro ObataEnergy Metabolism Group (C.A., C.P., M.R., J.T.v.D.) and Central Metabolism Group (T.O., A.R.F.), Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany;Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier-Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal (C.A.);Departamento de Produção Animal e Vegetal, Faculdade de Ciências Agrárias, Universidade Federal do Amazonas, Manaus, Amazonas 69082-653, Brazil (M.R.);University of Angers (H.D., A.M.L.) and Institut National de la Recherche Agronomique (A.M.L.), Unité Mixte de Recherche 1345 IRHS, SFR 4207 QUASAV, F-49045 Angers, France; andInstitute for Biology I, RWTH Aachen University, D-52056 Aachen, Germany (J.T.v.D.).ORCID http://orcid.org/0000-0001-8931-7722
Alisdair R FernieEnergy Metabolism Group (C.A., C.P., M.R., J.T.v.D.) and Central Metabolism Group (T.O., A.R.F.), Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany;Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier-Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal (C.A.);Departamento de Produção Animal e Vegetal, Faculdade de Ciências Agrárias, Universidade Federal do Amazonas, Manaus, Amazonas 69082-653, Brazil (M.R.);University of Angers (H.D., A.M.L.) and Institut National de la Recherche Agronomique (A.M.L.), Unité Mixte de Recherche 1345 IRHS, SFR 4207 QUASAV, F-49045 Angers, France; andInstitute for Biology I, RWTH Aachen University, D-52056 Aachen, Germany (J.T.v.D.).
Joost T van DongenEnergy Metabolism Group (C.A., C.P., M.R., J.T.v.D.) and Central Metabolism Group (T.O., A.R.F.), Max Planck Institute of Molecular Plant Physiology, D-14476 Potsdam-Golm, Germany;Plant Metabolomics Laboratory, Instituto de Tecnologia Química e Biológica António Xavier-Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal (C.A.);Departamento de Produção Animal e Vegetal, Faculdade de Ciências Agrárias, Universidade Federal do Amazonas, Manaus, Amazonas 69082-653, Brazil (M.R.);University of Angers (H.D., A.M.L.) and Institut National de la Recherche Agronomique (A.M.L.), Unité Mixte de Recherche 1345 IRHS, SFR 4207 QUASAV, F-49045 Angers, France; andInstitute for Biology I, RWTH Aachen University, D-52056 Aachen, Germany (J.T.v.D.).ORCID http://orcid.org/0000-0001-7944-9289
Max Planck Institute of Molecular Plant Physiology · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Based on enzyme activity assays and metabolic responses to waterlogging of the legume Lotus japonicus, it was previously suggested that, during hypoxia, the tricarboxylic acid cycle switches to a noncyclic operation mode. Hypotheses were postulated to explain the alternative metabolic pathways involved, but as yet, a direct analysis of the relative redistribution of label through the corresponding pathways was not made. Here, we describe the use of stable isotope-labeling experiments for studying metabolism under hypoxia using wild-type roots of the crop legume soybean (Glycine max). [(13)C]Pyruvate labeling was performed to compare metabolism through the tricarboxylic acid cycle, fermentation, alanine metabolism, and the γ-aminobutyric acid shunt, while [(13)C]glutamate and [(15)N]ammonium labeling were performed to address the metabolism via glutamate to succinate. Following these labelings, the time course for the redistribution of the (13)C/(15)N label throughout the metabolic network was evaluated with gas chromatography-time of flight-mass spectrometry. Our combined labeling data suggest the inhibition of the tricarboxylic acid cycle enzyme succinate dehydrogenase, also known as complex II of the mitochondrial electron transport chain, providing support for the bifurcation of the cycle and the down-regulation of the rate of respiration measured during hypoxic stress. Moreover, up-regulation of the γ-aminobutyric acid shunt and alanine metabolism explained the accumulation of succinate and alanine during hypoxia.

Indexed as

Carbon IsotopesCell RespirationCitric Acid Cyclegamma-Aminobutyric AcidGas Chromatography-Mass SpectrometryGlycine maxIsotope LabelingNitrogen IsotopesOxygenPlant ProteinsPlant RootsPyruvic AcidSuccinate DehydrogenaseCarbon Isotopesgamma-Aminobutyric AcidNitrogen IsotopesOxygenPlant ProteinsPyruvic AcidSuccinate Dehydrogenase

Identifiers

PMID26553649
PMCPMC4704563
OpenAlexW2132522144

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.