ArticleBMC plant biology2015
A bulk segregant transcriptome analysis reveals metabolic and cellular processes associated with Orange allelic variation and fruit β-carotene accumulation in melon fruit.
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 70 citations in OpenAlex.
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- Genome-Wide Identification and Chilling Stress Analysis of the NF-Y Gene Family in Melon.International journal of molecular sciences · 2023Article
- Transcriptome analysis reveals association of carotenoid metabolism pathway with fruit color in melon.Scientific reports · 2023Article
- R3-MYB repressor Mybr97 is a candidate gene associated with the Anthocyanin3 locus and enhanced anthocyanin accumulation in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- New Insights intoInternational journal of molecular sciences · 2022Article
- Transcription Factor CmNAC34 RegulatedInternational journal of molecular sciences · 2022Article
- Plant carotenoids: recent advances and future perspectives.Molecular horticulture · 2022Review
- Harnessing the potential of bulk segregant analysis sequencing and its related approaches in crop breeding.Frontiers in genetics · 2022Review
- Identification of Key Gene Networks Controlling Soluble Sugar and Organic Acid Metabolism During Oriental Melon Fruit Development by Integrated Analysis of Metabolic and Transcriptomic Analyses.Frontiers in plant science · 2022Article
- Multispecies transcriptomes reveal core fruit development genes.Frontiers in plant science · 2022Article
- Role of reactive oxygen species in lesion mimic formation and conferred basal resistance toFrontiers in plant science · 2022Article
- Multi-strategy engineering greatly enhances provitamin A carotenoid accumulation and stability in Arabidopsis seeds.aBIOTECH · 2021Article
- Comparative transcriptome analyses shed light on carotenoid production and plastid development in melon fruit.Horticulture research · 2021Article
- Transcriptome profiling of non-climacteric 'yellow' melon during ripening: insights on sugar metabolism.BMC genomics · 2020Article
- Genome-wide identification of microRNAs involved in the regulation of fruit ripening and climacteric stages in melon (Horticulture research · 2020Article
- The anther-specific CYP704B is potentially responsible for MSG26 male sterility in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
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Authors and funding
15 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMelon fruit flesh color is primarily controlled by the "golden" single nucleotide polymorhism of the "Orange" gene, CmOr, which dominantly triggers the accumulation of the pro-vitamin A molecule, β-carotene, in the fruit mesocarp. The mechanism by which CmOr operates is not fully understood. To identify cellular and metabolic processes associated with CmOr allelic variation, we compared the transcriptome of bulks of developing fruit of homozygous orange and green fruited F3 families derived from a cross between orange and green fruited parental lines.
resultsPooling together F3 families that share same fruit flesh color and thus the same CmOr allelic variation, normalized traits unrelated to CmOr allelic variation. RNA sequencing analysis of these bulks enabled the identification of differentially expressed genes. These genes were clustered into functional groups. The relatively enriched functional groups were those involved in photosynthesis, RNA and protein regulation, and response to stress.
conclusionsThe differentially expressed genes and the enriched processes identified here by bulk segregant RNA sequencing analysis are likely part of the regulatory network of CmOr. Our study demonstrates the resolution power of bulk segregant RNA sequencing in identifying genes related to commercially important traits and provides a useful tool for better understanding the mode of action of CmOr gene in the mediation of carotenoid accumulation.
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