Evidence map›Paper›PMID 26552967›Full record

ArticleMedical science monitor : international medical journal of experimental and clinical research2015

2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL.

Yuping Zhang, Feizhou Huang, Jian Wang, Hongwu Luo, Zhichao Wang

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Medical science monitor : international medical journal of experimental and clinical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Review
  6. Characterization of metabolic landscape in hepatocellular carcinoma.World journal of gastrointestinal oncology · 2021
    Review
  7. Article
  8. 2-Deoxy-d-Glucose and Its Analogs: From Diagnostic to Therapeutic Agents.International journal of molecular sciences · 2019
    Review
  9. Review
  10. Article
  11. Review
  12. FLIP(L) is critical for aerobic glycolysis in hepatocellular carcinoma.Journal of experimental & clinical cancer research : CR · 2016
    Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yuping ZhangDepartment of Normal Surgical, The 3rd Xiangya Hospital of Central South University, Changsha, Hunan, China (mainland).
Feizhou HuangDepartment of Normal Surgical, The 3rd Xiangya Hospital of Central South University, Changsha, Hunan, China (mainland).
Jian WangDepartment of Normal Surgical, Genetics Research Room of Central South University, Changsha, Hunan, China (mainland).
Hongwu LuoDepartment of Normal Surgical, The 3rd Xiangya Hospital of Central South University, Changsha, Hunan, China (mainland).
Zhichao WangDepartment of Normal Surgical, The 3rd Xiangya Hospital of Central South University, Changsha, Hunan, China (mainland).
Central South University · CNXiangya Hospital Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCancer cells survival depends on glucose metabolism and ATP. Inhibiting glucose metabolism is a possible anticancer treatment. The phosphorylation of 2-deoxy-D-glucose (2-DG), which is a glycogen analogue, seriously affects the normal glycometabolism phosphorylation process, leading to ATP consumption. Studies showed that 2-DG could regulate RIP and c-FLIP. This paper aimed to investigate the effect of 2-DG on RIP and c-FLIP expression in HepG2 and Hep3B cells, further illustrating the effect and mechanism of 2-DG regulating RIP and c-FLIP expression on liver cancer cell apoptosis induced by TRAIL. MATERIAL AND

methodsRIP and c-FLIP gene silencing HepG2 and Hep3B cell models were established by siRNA and detected by Western blot. Cell viability was determined by MTT and apoptosis rate was measured by flow cytometry. JC-1 fluorescent probe was used to test mitochondrial membrane potential.

results2-DG or TRAIL alone significantly reduced HepG2 and Hep3B cell survival rate and promoted apoptosis. Compared with the single TRAIL treatment group, the combination of 2-DG and TRAIL could reduce cell survival rate, increase apoptosis rate, and decease mitochondrial membrane potential, which is dependent on Caspases. 2-DG can inhibit RIP and c-FLIP expression, leading to increased TRAIL-induced HepG2 and Hep3B cells apoptosis.

conclusions2-DG can down-regulate RIP and c-FLIP expression, and change Caspases activities to increase the liver cancer cell apoptosis induced by TRAIL.

Indexed as

ApoptosisAdenosine TriphosphateCASP8 and FADD-Like Apoptosis Regulating ProteinDeoxyglucoseFlow CytometryGene SilencingHep G2 CellsHumansLiver NeoplasmsMembrane PotentialsMitochondriaReceptor-Interacting Protein Serine-Threonine KinasesRNA, Small InterferingTNF-Related Apoptosis-Inducing LigandAdenosine TriphosphateCASP8 and FADD-Like Apoptosis Regulating ProteinCFLAR protein, humanDeoxyglucoseReceptor-Interacting Protein Serine-Threonine KinasesRNA, Small InterferingTNF-Related Apoptosis-Inducing LigandTNFSF10 protein, human

Identifiers

PMID26552967
PMCPMC4646230
OpenAlexW2251773668

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.