ArticleJournal of pediatric surgery2016
Effect of cell seeding on neotissue formation in a tissue engineered trachea.
Article in Journal of pediatric surgery, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Applications of Electrospinning for Tissue Engineering in Otolaryngology.The Annals of otology, rhinology, and laryngology · 2021Pooled it
- Towards a bioengineered airway: advances in tracheal tissue engineering and biofabrication.Frontiers in cell and developmental biology · 2026Review
- Advancements in lung regeneration: from bench to bedside.Journal of translational medicine · 2025Review
- Tracheal Tissue Engineering: Principles and State of the Art.Bioengineering (Basel, Switzerland) · 2024Review
- Isolation of multipotent progenitor cells from pleura and pericardium for tracheal tissue engineering purposes.Journal of cellular and molecular medicine · 2021Article
- Tissue engineering applications in otolaryngology-The state of translation.Laryngoscope investigative otolaryngology · 2020Review
- Deconstructing tissue engineered trachea: Assessing the role of synthetic scaffolds, segmental replacement and cell seeding on graft performance.Acta biomaterialia · 2020Article
- Factors Influencing Poor Outcomes in Synthetic Tissue-Engineered Tracheal Replacement.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2019Article
- Electrospun scaffolds limit the regenerative potential of the airway epithelium.Laryngoscope investigative otolaryngology · 2019Article
- Mouse Model of Tracheal Replacement With Electrospun Nanofiber Scaffolds.The Annals of otology, rhinology, and laryngology · 2019Article
- Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model.Journal of visualized experiments : JoVE · 2019Article
- Quantification of tissue-engineered trachea performance with computational fluid dynamics.The Laryngoscope · 2018Article
- Designing a tissue-engineered tracheal scaffold for preclinical evaluation.International journal of pediatric otorhinolaryngology · 2018Article
- Endoscopic management of tissue-engineered tracheal graft stenosis in an ovine model.The Laryngoscope · 2017Article
- The innate immune response to lower respiratory tract E. Coli infection and the role of the CCL2-CCR2 axis in neonatal mice.Cytokine · 2017Article
- Autologous Cell Seeding in Tracheal Tissue Engineering.Current stem cell reports · 2017Review
- Clinical Translation of Tissue Engineered Trachea Grafts.The Annals of otology, rhinology, and laryngology · 2016Review
- Improved cellular infiltration in electrospun fiber via engineered porosity.Tissue engineering · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
backgroundSurgical management of long segment tracheal disease is limited by a paucity of donor tissue and poor performance of synthetic materials. A potential solution is the development of a tissue-engineered tracheal graft (TETG) which promises an autologous airway conduit with growth capacity.
methodsWe created a TETG by vacuum seeding bone marrow-derived mononuclear cells (BM-MNCs) on a polymeric nanofiber scaffold. First, we evaluated the role of scaffold porosity on cell seeding efficiency in vitro. We then determined the effect of cell seeding on graft performance in vivo using an ovine model.
resultsSeeding efficiency of normal porosity (NP) grafts was significantly increased when compared to high porosity (HP) grafts (NP: 360.3 ± 69.19 × 10(3) cells/mm(2); HP: 133.7 ± 22.73 × 10(3) cells/mm(2); p<0.004). Lambs received unseeded (n=2) or seeded (n=3) NP scaffolds as tracheal interposition grafts for 6 weeks. Three animals were terminated early owing to respiratory complications (n=2 unseeded, n=1 seeded). Seeded TETG explants demonstrated wound healing, epithelial migration, and delayed stenosis when compared to their unseeded counterparts.
conclusionVacuum seeding BM-MNCs on nanofiber scaffolds for immediate implantation as tracheal interposition grafts is a viable approach to generate TETGs, but further preclinical research is warranted before advocating this technology for clinical application.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.