ReviewMethods in molecular biology (Clifton, N.J.)2016
Guidelines for the Use of Protein Domains in Acidic Phospholipid Imaging.
Review in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Plant PI-PLC signaling in stress and development.Plant physiology · 2025Review
- Probing and imaging phospholipid dynamics in live cells.Life metabolism · 2024Review
- Syntaxin 17 recruitment to mature autophagosomes is temporally regulated by PI4P accumulation.eLife · 2024Article
- FAB1C, a phosphatidylinositol 3-phosphate 5-kinase, interacts with PIN-FORMEDs and modulates their lytic trafficking in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- TheeLife · 2022Article
- The functions of phospholipases and their hydrolysis products in plant growth, development and stress responses.Progress in lipid research · 2022Review
- Imaging the living plant cell: From probes to quantification.The Plant cell · 2022Review
- Bioimaging tools move plant physiology studies forward.Frontiers in plant science · 2022Article
- Exogenous treatment of Arabidopsis seedlings with lyso-phospholipids for the inducible complementation of lipid mutants.STAR protocols · 2021Article
- Anionic phospholipid gradients: an uncharacterized frontier of the plant endomembrane network.Plant physiology · 2021Article
- Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells.Lipids in health and disease · 2020Article
- Specific Recruitment of Phosphoinositide Species to the Plant-Pathogen Interfacial Membrane Underlies Arabidopsis Susceptibility to Fungal Infection.The Plant cell · 2020Article
- Identification of salicylic acid-independent responses in an Arabidopsis phosphatidylinositol 4-kinase beta double mutant.Annals of botany · 2020Article
- Lipidomics Reveals the Therapeutic Effects of EtOAc Extract ofFrontiers in pharmacology · 2020Article
- Greasing the Wheels of Lipid Biology with Chemical Tools.Trends in biochemical sciences · 2018Review
- Article
- Golgi-Associated Protein Kinase C-ε Is Delivered to Phagocytic Cups: Role of Phosphatidylinositol 4-Phosphate.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Quantifying lipid changes in various membrane compartments using lipid binding protein domains.Cell calcium · 2017Review
- Anionic lipids and the maintenance of membrane electrostatics in eukaryotes.Plant signaling & behavior · 2017Article
- Brassinosteroid signaling and BRI1 dynamics went underground.Current opinion in plant biology · 2016Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Acidic phospholipids are minor membrane lipids but critically important for signaling events. The main acidic phospholipids are phosphatidylinositol phosphates (PIPs also known as phosphoinositides), phosphatidylserine (PS), and phosphatidic acid (PA). Acidic phospholipids are precursors of second messengers of key signaling cascades or are second messengers themselves. They regulate the localization and activation of many proteins, and are involved in virtually all membrane trafficking events. As such, it is crucial to understand the subcellular localization and dynamics of each of these lipids within the cell. Over the years, several techniques have emerged in either fixed or live cells to analyze the subcellular localization and dynamics of acidic phospholipids. In this chapter, we review one of them: the use of genetically encoded biosensors that are based on the expression of specific lipid binding domains (LBDs) fused to fluorescent proteins. We discuss how to design such sensors, including the criteria for selecting the lipid binding domains of interest and to validate them. We also emphasize the care that must be taken during data analysis as well as the main limitations and advantages of this approach.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.