ArticleJournal of assisted reproduction and genetics2016
What maintains the high intra-follicular estradiol concentration in pre-ovulatory follicles?
Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Article
- Dysfunction of Human Estrogen Signaling as a Novel Molecular Signature of Polycystic Ovary Syndrome.International journal of molecular sciences · 2023Article
- Estrogenic and Non-Estrogenic Disruptor Effect of Zearalenone on Male Reproduction: A Review.International journal of molecular sciences · 2023Review
- Transcriptome Analysis of the Ovaries of Taihe Black-Bone Silky Fowls at Different Egg-Laying Stages.Genes · 2022Article
- Ovarian follicular function is not altered by SARS-CoV-2 infection or BNT162b2 mRNA COVID-19 vaccination.Human reproduction (Oxford, England) · 2021Article
- Rescue of caprine fetal ovaries, vitrification and follicular development after xenotransplantation in two immunodeficient mice models.Animal reproduction · 2020Article
- A Draft Map of the Human Ovarian Proteome for Tissue Engineering and Clinical Applications.Molecular & cellular proteomics : MCP · 2019Article
- Estradiol and Progesterone Induced Differentiation and Increased Stemness Gene Expression of Human Fallopian Tube Epithelial Cells.Journal of Cancer · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of the study was to establish the mechanism by which the estrogen concentration difference between the follicular fluid and the serum is maintained.
methodsWe used dialysis membrane with a pore size of <3 KD to characterize the estrogen-binding capacity of the follicular fluid. We performed PCR, western blot, and ELISA on luteinized granulosa cells to determine if sex hormone-binding globulin (SHBG) is produced by granulosa cells, and finally we used affinity columns and mass spectrometry to identify the estrogen-binding protein in the follicular fluid.
resultsWe found that a significant estrogen concentration difference is maintained in a cell-free system and is lost with proteolysis of the follicular fluid proteins. Luteinized granulosa cells are likely not a source of SHBG, as we were not able to detect expression of SHBG in these cells. Perlecan was the most highly enriched follicular fluid protein in the affinity columns.
conclusionsWe were able to identify perlecan as the most likely candidate for the major estrogen-binding protein in the follicular fluid.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.