ArticleProteomics2016
Micro-proteomics with iterative data analysis: Proteome analysis in C. elegans at the single worm level.
Article in Proteomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 39 citations in OpenAlex.
- Integrating MACSPI and SILAC for Neuron Type-specific Proteomics in Caenorhabditis elegans.Molecular & cellular proteomics : MCP · 2026Article
- Caenorhabditis elegans as an experimental model for resilience-boosting microbiota interventions in nonmodel ectotherms.FEMS microbiology reviews · 2026Review
- Detecting gene expression in Caenorhabditis elegans.Genetics · 2025Review
- Digital Microfluidics for Sample Preparation in Low-Input Proteomics.Small methods · 2025Review
- Single-tissue proteomics inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Single-worm quantitative proteomics reveals aging heterogeneity in isogenic Caenorhabditis elegans.Aging cell · 2024Article
- Elegant Nematodes Improve Our Understanding of Human Neuronal Diseases, the Role of Pollutants and Strategies of Resilience.Environmental science & technology · 2023Review
- Cell-Type-Specific Neuroproteomics of Synapses.Biomolecules · 2023Review
- Identification of Important Factors Causing Developmental Arrest in Cloned Pig Embryos by Embryo Biopsy Combined with Microproteomics.International journal of molecular sciences · 2022Article
- Exposome, Molecular Pathways and One Health: The InvertebrateInternational journal of molecular sciences · 2022Review
- Identification of putative reader proteins of 5-methylcytosine and its derivatives inWellcome open research · 2022Article
- Quantitative proteomics of small numbers of closely-related cells: Selection of the optimal method for a clinical setting.Frontiers in medicine · 2022Article
- Proteomics for Low Cell Numbers: How to Optimize the Sample Preparation Workflow for Mass Spectrometry Analysis.Journal of proteome research · 2021Article
- Quo VadisMetabolites · 2021Review
- DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans.Nature communications · 2020Article
- Laser microdissection-based microproteomics of the hippocampus of a rat epilepsy model reveals regional differences in protein abundances.Scientific reports · 2020Article
- Application of a Sensitive and Reproducible Label-Free Proteomic Approach to Explore the Proteome of Individual Meiotic-Phase Barley Anthers.Frontiers in plant science · 2019Article
- Measuring and modeling interventions in aging.Current opinion in cell biology · 2018Review
- Of dogs and hookworms: man's best friend and his parasites as a model for translational biomedical research.Parasites & vectors · 2018Review
- The Encyclopedia of Proteome Dynamics: a big data ecosystem for (prote)omics.Nucleic acids research · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Proteomics studies typically analyze proteins at a population level, using extracts prepared from tens of thousands to millions of cells. The resulting measurements correspond to average values across the cell population and can mask considerable variation in protein expression and function between individual cells or organisms. Here, we report the development of micro-proteomics for the analysis of Caenorhabditis elegans, a eukaryote composed of 959 somatic cells and ∼1500 germ cells, measuring the worm proteome at a single organism level to a depth of ∼3000 proteins. This includes detection of proteins across a wide dynamic range of expression levels (>6 orders of magnitude), including many chromatin-associated factors involved in chromosome structure and gene regulation. We apply the micro-proteomics workflow to measure the global proteome response to heat-shock in individual nematodes. This shows variation between individual animals in the magnitude of proteome response following heat-shock, including variable induction of heat-shock proteins. The micro-proteomics pipeline thus facilitates the investigation of stochastic variation in protein expression between individuals within an isogenic population of C. elegans. All data described in this study are available online via the Encyclopedia of Proteome Dynamics (http://www.peptracker.com/epd), an open access, searchable database resource.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.