ArticleNature methods2016
Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe.
Article in Nature methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 139 papers.
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Who cites it
139 citing papers in PubMed, 267 citations in OpenAlex.
- Near-infrared photochromic miniprotein enables photoacoustic molecular imaging of acute inflammation and intracellular antigens.Nature methods · 2026Article
- Advanced methods of wearable ultrasound and photoacoustic imaging devices: A review.Photoacoustics · 2026Article
- Photoacoustic and ultrasound Imaging of placental functions in gestational diabetes: From diagnosis to precision interventions.Photoacoustics · 2026Review
- Deep learning for optoacoustic imaging of reversibly switchable proteins: training and performance testing using simulated multispectral optoacoustic tomography images.Journal of biomedical optics · 2026Article
- Activatable smart contrast agents for photoacoustic imaging.Smart molecules : open access · 2026Review
- All-fiber photoacoustic endomicroscopy reveals layer-specific angiogenesis-oxygenation uncoupling in experimental colitis.Science advances · 2026Article
- 3D-PAULM: Integrated Photoacoustic Tomography and Ultrasound Localization Microscopy for Multiparametric Brain and Tumor Imaging.bioRxiv : the preprint server for biology · 2026Article
- Advanced imaging techniques for tumor intraoperative navigation imaging.Npj imaging · 2026Review
- Photoacoustic Microscopy for Multiscale Biological System Visualization and Clinical Translation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Clinical translation of photoacoustic imaging using exogenous molecular contrast agents [Invited].Biomedical optics express · 2025Review
- Recent advancements in molecular photoacoustic tomography.JPhys photonics · 2025Review
- Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout.Nature communications · 2025Article
- Article
- Non-Invasive Deep-Brain Imaging With 3D Integrated Photoacoustic Tomography and Ultrasound Localization Microscopy (3D-PAULM).IEEE transactions on medical imaging · 2025Article
- Three-dimensional diffractive acoustic tomography.Nature communications · 2025Article
- Large-scale super-resolution optoacoustic imaging facilitated by FeNP/ICG-loaded coreless polyelectrolyte microcapsules.Theranostics · 2025Article
- Emerging Photoacoustic Imaging Techniques for Peripheral Arterial Disease.Current treatment options in cardiovascular medicine · 2025Review
- X-ray-induced acoustic computed tomography: 3D X-ray absorption imaging from a single view.Science advances · 2024Article
- Sound Out the Deep Clarity: Super-Resolution Photoacoustic Imaging at Depths.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2024Review
- Enhanced dual-mode imaging: Superior photoacoustic and ultrasound endoscopy in live pigs using a transparent ultrasound transducer.Science advances · 2024Article
79 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Photoacoustic tomography (PAT) of genetically encoded probes allows for imaging of targeted biological processes deep in tissues with high spatial resolution; however, high background signals from blood can limit the achievable detection sensitivity. Here we describe a reversibly switchable nonfluorescent bacterial phytochrome for use in multiscale photoacoustic imaging, BphP1, with the most red-shifted absorption among genetically encoded probes. BphP1 binds a heme-derived biliverdin chromophore and is reversibly photoconvertible between red and near-infrared light-absorption states. We combined single-wavelength PAT with efficient BphP1 photoswitching, which enabled differential imaging with substantially decreased background signals, enhanced detection sensitivity, increased penetration depth and improved spatial resolution. We monitored tumor growth and metastasis with ∼ 100-μm resolution at depths approaching 10 mm using photoacoustic computed tomography, and we imaged individual cancer cells with a suboptical-diffraction resolution of ∼ 140 nm using photoacoustic microscopy. This technology is promising for biomedical studies at several scales.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.