Evidence map›Paper›PMID 26550599›Full record

ArticleData in brief2015

Differential cytotoxicity of long-chain bases for human oral gingival epithelial keratinocytes, oral fibroblasts, and dendritic cells.

Leslie A Mehalick, Christopher Poulsen, Carol L Fischer, Emily A Lanzel, Amber M Bates, Katherine S Walters, Joseph E Cavanaugh, Janet M Guthmiller, Georgia K Johnson, Philip W Wertz and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Data in brief, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Leslie A MehalickDepartment of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
Christopher PoulsenDepartment of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
Carol L FischerDows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
Emily A LanzelDepartment of Oral Pathology, Radiology and Medicine, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
Amber M BatesDows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
Katherine S WaltersCentral Microscopy Research Facility, The University of Iowa, Iowa City, IA 52242, USA.
Joseph E CavanaughDepartment of Biostatistics, College of Public Health, The University of Iowa, Iowa City, IA 52242, USA.
Janet M GuthmillerCollege of Dentistry, University of Nebraska Medical Center, Lincoln, NE 68583, USA.
Georgia K JohnsonDepartment of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
Philip W WertzDows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA ; Department of Oral Pathology, Radiology and Medicine, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
Kim A BrogdenDepartment of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA ; Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USA.
University of Iowa · USUniversity of Nebraska Medical Center · US

Funding

University of Iowa Institutional Training Program in Oral Health ResearchT90DE023520 · NIDCR · UNIVERSITY OF IOWA · PI STEVEN M. LEVY · 2013 to 2026
$5.6M
Defensin-induced Adaptive Immunity to HagBR01DE014390 · NIDCR · UNIVERSITY OF IOWA · PI BROGDEN, KIM A · 2003 to 2014
$3.4M
University of Iowa Institutional Training Program in Oral Health ResearchR90DE024296 · NIDCR · UNIVERSITY OF IOWA · PI STEVEN M. LEVY · 2013 to 2026
$1.8M
NIDCR NIH HHS R01 DE014390NIDCR NIH HHS R90 DE024296NIDCR NIH HHS T90 DE023520
6 · The paper itself

Abstract

Long-chain bases, found in the oral cavity, have potent antimicrobial activity against oral pathogens. In an article associated with this dataset, Poulson and colleagues determined the cytotoxicities of long-chain bases (sphingosine, dihydrosphingosine, and phytosphingosine) for human oral gingival epithelial (GE) keratinocytes, oral gingival fibroblasts (GF), dendritic cells (DC), and squamous cell carcinoma (SCC) cell lines [1]. Poulson and colleagues found that GE keratinocytes were more resistant to long-chain bases as compared to GF, DC, and SCC cell lines [1]. In this study, we assess the susceptibility of DC to lower concentrations of long chain bases. 0.2-10.0 µM long-chain bases and GML were not cytotoxic to DC; 40.0-80.0 µM long-chain bases, but not GML, were cytotoxic for DC; and 80.0 µM long-chain bases were cytotoxic to DC and induced cellular damage and death in less than 20 mins. Overall, the LD50 of long-chain bases for GE keratinocytes, GF, and DC were considerably higher than their minimal inhibitory concentrations for oral pathogens, a finding important to pursuing their future potential in treating periodontal and oral infections.

Indexed as

CytotoxicityDendritic cellsDihydrosphingosineGlycerol monolaurateHuman oral gingival epithelial keratinocytesOral gingival fibroblastsOral squamous cell carcinoma cellsPhytosphingosineSphingosine

Identifiers

PMID26550599
PMCPMC4596918
OpenAlexW1511665425

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.