ArticleProtein science : a publication of the Protein Society2016
Rational design of a monomeric and photostable far-red fluorescent protein for fluorescence imaging in vivo.
Article in Protein science : a publication of the Protein Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
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- Microtubule polarity is instructive for many aspects of neuronal polarity.Developmental biology · 2022Article
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division.Science advances · 2021Article
- Comparative Analysis of Bacteriophytochrome Agp2 and Its Engineered Photoactivatable NIR Fluorescent Proteins PAiRFP1 and PAiRFP2.Biomolecules · 2020Article
- Structure-guided evolution of Green2 toward photostability and quantum yield enhancement by F145Y substitution.Protein science : a publication of the Protein Society · 2020Article
- Kinetochore proteins suppress neuronal microtubule dynamics and promote dendrite regeneration.Molecular biology of the cell · 2020Article
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- Bacteriophytochromes - from informative model systems of phytochrome function to powerful tools in cell biology.Current opinion in structural biology · 2019Review
- A Bright Future for Fluorescence Imaging of Fungi in Living Hosts.Journal of fungi (Basel, Switzerland) · 2019Review
- Combining near-infrared fluorescence with Brainbow to visualize expression of specific genes within a multicolor context.Molecular biology of the cell · 2019Article
- Near-Infrared Fluorescent Proteins Engineered from Bacterial Phytochromes in Neuroimaging.Biophysical journal · 2017Article
- Designing brighter near-infrared fluorescent proteins: insights from structural and biochemical studies.Chemical science · 2017Article
- The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.Trends in biochemical sciences · 2017Review
- Advances in Imaging Techniques and Genetically Encoded Probes for Photoacoustic Imaging.Theranostics · 2016Review
Corrections and comments
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Authors and funding
14 authors at 9 institutions in 4 countries.
Funding
Abstract
Fluorescent proteins (FPs) are powerful tools for cell and molecular biology. Here based on structural analysis, a blue-shifted mutant of a recently engineered monomeric infrared fluorescent protein (mIFP) has been rationally designed. This variant, named iBlueberry, bears a single mutation that shifts both excitation and emission spectra by approximately 40 nm. Furthermore, iBlueberry is four times more photostable than mIFP, rendering it more advantageous for imaging protein dynamics. By tagging iBlueberry to centrin, it has been demonstrated that the fusion protein labels the centrosome in the developing zebrafish embryo. Together with GFP-labeled nucleus and tdTomato-labeled plasma membrane, time-lapse imaging to visualize the dynamics of centrosomes in radial glia neural progenitors in the intact zebrafish brain has been demonstrated. It is further shown that iBlueberry can be used together with mIFP in two-color protein labeling in living cells and in two-color tumor labeling in mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.