ArticleJournal of assisted reproduction and genetics2016
Endometrial microbiome at the time of embryo transfer: next-generation sequencing of the 16S ribosomal subunit.
Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 148 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
148 citing papers in PubMed, 2 syntheses or guidelines pooled it, 270 citations in OpenAlex.
- A bibliometric analysis of the frontiers and hotspots in chronic endometritis.Frontiers in medicine · 2025Pooled it
- The Role of Genital Tract Microbiome in Fertility: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Pregnancy outcomes after vaginal probiotic supplementation before frozen embryo transfer: a randomized controlled study.Scientific reports · 2023Trial
- Implantation Outcome-Specific Associations Between Stromal Senescence and the Endometrial Microbiota.Microorganisms · 2026Article
- Exploring the Possible Role of Endometriosis-Associated Dysbiosis in Endometrial Carcinogenesis.Medicina (Kaunas, Lithuania) · 2026Review
- Unexplained Recurrent Pregnancy Loss and Emerging Endometrial Biology: A Clinically Oriented Narrative Review.Cureus · 2026Review
- The role of the microbiome in uterine cancer: insights into tumorigenesis, therapeutic implications, and clinical prospects.Journal of the Egyptian National Cancer Institute · 2026Review
- Maternal Microbiome in Fetal Programming: A One Health Perspective on Translational Implications for Early-Life Health.Microorganisms · 2026Review
- Microbiological Profiling of Menstrual Blood Aspirated from the Uterus in Patients Undergoing Frozen Embryo Transfer.Diagnostics (Basel, Switzerland) · 2026Article
- Beyond the Barrier: Epithelial Cells as Immune Sentinels in the Female Genital Tract.American journal of reproductive immunology (New York, N.Y. : 1989) · 2026Review
- Bacterial Extracellular Vesicles: New Hype or Hope to Explain Reproductive Host-Microbiota Interactions.Journal of extracellular vesicles · 2026Review
- Trimethylamine-producing microbe Bacillus megaterium KCTC 3007 promotes antitumor immunity in endometrial cancer via type I interferon response pathways.Microbiome · 2026Article
- The Role of Endometrial Microbiota in the Pathogenesis of Chronic Endometritis: A Systematic Review and Meta-Analysis.Biomedicines · 2026Review
- Contribution of endometrial microbiome to inflammation-mediated infertility in women undergoing ART.Human reproduction (Oxford, England) · 2026Article
- Precision Microbial Therapeutics for Infertility: Next-Generation Probiotics, Engineered Biologics and Translational Pathways.Microbial biotechnology · 2026Review
- Efficacy of endometrial microbiota testing-guided personalized therapy in infertile women stratified by CD138 status: a retrospective cohort study.Frontiers in endocrinology · 2026Article
- Dual Role of the Endometrial Microbiome-Immune Axis: From Endometrial Homeostasis to Reproductive Disorders.International journal of women's health · 2026Review
- Phenotype- and phase-directed immune interpretation in recurrent implantation failure and pregnancy loss: an integrative framework.Frontiers in reproductive health · 2026Review
- Vaginal Nugent Lactobacillus morphotype score and endometrial pathogen detection: a two-centre retrospective study.Frontiers in endocrinology · 2026Observational
- Uterine-cavity Lactobacillus relative abundance and endometrial polyp recurrence after hysteroscopic polypectomy: a retrospective cohort study.Frontiers in public health · 2026Article
88 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeCharacterization of the human microbiome has become more precise with the application of powerful molecular tools utilizing the unique 16S ribosomal subunit's hypervariable regions to greatly increase sensitivity. The microbiome of the lower genital tract can prognosticate obstetrical outcome while the upper reproductive tract remains poorly characterized. Here, the endometrial microbiome at the time of single embryo transfer (SET) is characterized by reproductive outcome.
methodsConsecutive patients undergoing euploid, SET was included in the analysis. After embryo transfer, performed as per routine, the most distal 5-mm portion of the transfer catheter was sterilely placed in a DNA free PCR tube. Next-generation sequencing of the bacteria specific 16S ribosome gene was performed, allowing genus and species calls for microorganisms.
resultsTaxonomy assignments were made on 35 samples from 33 patients and 2 Escherichia coli controls. Of the 33 patients, 18 had ongoing pregnancies and 15 did not. There were a total of 278 different genus calls present across patient samples. The microbiome at time of transfer for those patients with ongoing pregnancy vs. those without ongoing pregnancy was characterized by top genera by sum fraction. Lactobacillus was the top species call for both outcomes.
conclusionsThe data presented here show the microbiome at the time of embryo transfer can successfully be characterized without altering standard clinical practice. This novel approach, both in specimen collection and analysis, is the first step toward the goal of determining physiologic from pathophysiologic microbiota. Further studies will help delineate if differences in the microbiome at the time of embryo transfer have a reliable impact on pregnancy outcome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.