Evidence map›Paper›PMID 26547127›Full record

ArticleOral oncology2016

Electronic cigarettes induce DNA strand breaks and cell death independently of nicotine in cell lines.

Vicky Yu, Mehran Rahimy, Avinaash Korrapati, Yinan Xuan, Angela E Zou, Aswini R Krishnan, Tzuhan Tsui, Joseph A Aguilera, Sunil Advani, Laura E Crotty Alexander and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oral oncology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
141citing papers in PubMed, 4 pooled it
13.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

141 citing papers in PubMed, 4 syntheses or guidelines pooled it, 259 citations in OpenAlex.

  1. Pooled it
  2. Lung Damage Caused by Heated Tobacco Products and Electronic Nicotine Delivery Systems: A Systematic Review.International journal of environmental research and public health · 2021
    Pooled it
  3. Pooled it
  4. Overview of Electronic Nicotine Delivery Systems: A Systematic Review.American journal of preventive medicine · 2017
    Pooled it
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81 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Vicky YuDepartment of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, United States.
Mehran RahimyDepartment of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, United States.
Avinaash KorrapatiDepartment of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, United States.
Yinan XuanDepartment of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, United States.
Angela E ZouDepartment of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, United States.
Aswini R KrishnanDepartment of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, United States.
Tzuhan TsuiDivision of Pulmonary and Critical Care, University of California, San Diego, La Jolla, California, United States.
Joseph A AguileraDepartment of Radiation Medicine and Applied Sciences, Moores Cancer Center, University of California, San Diego, La Jolla, California, United States.
Sunil AdvaniDepartment of Radiation Medicine and Applied Sciences, Moores Cancer Center, University of California, San Diego, La Jolla, California, United States.
Laura E Crotty AlexanderDivision of Pulmonary and Critical Care, University of California, San Diego, La Jolla, California, United States; Pulmonary and Critical Care Section, VA San Diego Healthcare System, La Jolla, California, United States.
Kevin T BrumundDepartment of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, United States.
Jessica Wang-RodriguezDepartment of Pathology, VA San Diego Healthcare System, La Jolla, California, United States.
Weg M OngkekoDepartment of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, United States.
University of California San Diego · USVA San Diego Healthcare System · US

Funding

VirologyP30AI036214 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SUSAN JANET LITTLE · 1994 to 2026
$78.4M
Non-coding RNAs in salivary gland tumorR21DE023242 · NIDCR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ONGKEKO, RUTHERFORD M · 2012 to 2013
$417k
Genetic and Pharmacologic Evaluation of the Role of HIF-1a in asthmaIK2BX001313 · VA · VA SAN DIEGO HEALTHCARE SYSTEM · PI CROTTY ALEXANDER, LAURA ELISE · 2011 to 2015
–
BLRD VA IK2 BX001313NIAID NIH HHS 30 AI036214NIAID NIH HHS P30 AI036214NIDCR NIH HHS DE023242NIDCR NIH HHS R21 DE023242
6 · The paper itself

Abstract

objectivesEvaluate the cytotoxicity and genotoxicity of short- and long-term e-cigarette vapor exposure on a panel of normal epithelial and head and neck squamous cell carcinoma (HNSCC) cell lines. MATERIALS AND

methodsHaCaT, UMSCC10B, and HN30 were treated with nicotine-containing and nicotine-free vapor extract from two popular e-cigarette brands for periods ranging from 48 h to 8 weeks. Cytotoxicity was assessed using Annexin V flow cytometric analysis, trypan blue exclusion, and clonogenic assays. Genotoxicity in the form of DNA strand breaks was quantified using the neutral comet assay and γ-H2AX immunostaining.

resultsE-cigarette-exposed cells showed significantly reduced cell viability and clonogenic survival, along with increased rates of apoptosis and necrosis, regardless of e-cigarette vapor nicotine content. They also exhibited significantly increased comet tail length and accumulation of γ-H2AX foci, demonstrating increased DNA strand breaks.

conclusionE-cigarette vapor, both with and without nicotine, is cytotoxic to epithelial cell lines and is a DNA strand break-inducing agent. Further assessment of the potential carcinogenic effects of e-cigarette vapor is urgently needed.

Indexed as

Carcinoma, Squamous CellCell DeathCell Line, TumorDNA DamageElectronic Nicotine Delivery SystemsEpithelial CellsHead and Neck NeoplasmsHumansNicotineNicotinic AgonistsVolatilizationNicotineNicotinic AgonistsDNA damageElectronic cigarettesHead and neck squamous cell carcinoma (HNSCC)NicotineOral cancerSmokingStrand breaks

Identifiers

PMID26547127
PMCPMC4891196
OpenAlexW2104213999

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.