Evidence map›Paper›PMID 26546667›Full record

ArticlePlant physiology2016

The EXS Domain of PHO1 Participates in the Response of Shoots to Phosphate Deficiency via a Root-to-Shoot Signal.

Stefanie Wege, Ghazanfar Abbas Khan, Ji-Yul Jung, Evangelia Vogiatzaki, Sylvain Pradervand, Isabel Aller, Andreas J Meyer, Yves Poirier

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.

0numbers the graph read from it
0cells of the map it votes in
73citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

73 citing papers in PubMed.

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  16. Evolution of phosphate scouting in the terrestrial biosphere.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024
    Review
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13 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Stefanie WegeDepartment for Plant Molecular Biology (S.W., G.A.K., J.-Y.J., E.V., Y.P.) and Genomic Technologies Facility, Center for Integrative Genomics (S.P.), University of Lausanne, 1015 Lausanne, Switzerland;Vital-IT, Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland (S.P.); andInstitute for Crop Science and Natural Resources, Chemical Signaling, University of Bonn, 53113 Bonn, Germany (I.A., A.J.M.).ORCID http://orcid.org/0000-0002-7232-5889
Ghazanfar Abbas KhanDepartment for Plant Molecular Biology (S.W., G.A.K., J.-Y.J., E.V., Y.P.) and Genomic Technologies Facility, Center for Integrative Genomics (S.P.), University of Lausanne, 1015 Lausanne, Switzerland;Vital-IT, Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland (S.P.); andInstitute for Crop Science and Natural Resources, Chemical Signaling, University of Bonn, 53113 Bonn, Germany (I.A., A.J.M.).ORCID http://orcid.org/0000-0002-7557-6210
Ji-Yul JungDepartment for Plant Molecular Biology (S.W., G.A.K., J.-Y.J., E.V., Y.P.) and Genomic Technologies Facility, Center for Integrative Genomics (S.P.), University of Lausanne, 1015 Lausanne, Switzerland;Vital-IT, Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland (S.P.); andInstitute for Crop Science and Natural Resources, Chemical Signaling, University of Bonn, 53113 Bonn, Germany (I.A., A.J.M.).
Evangelia VogiatzakiDepartment for Plant Molecular Biology (S.W., G.A.K., J.-Y.J., E.V., Y.P.) and Genomic Technologies Facility, Center for Integrative Genomics (S.P.), University of Lausanne, 1015 Lausanne, Switzerland;Vital-IT, Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland (S.P.); andInstitute for Crop Science and Natural Resources, Chemical Signaling, University of Bonn, 53113 Bonn, Germany (I.A., A.J.M.).ORCID http://orcid.org/0000-0003-2503-316X
Sylvain PradervandDepartment for Plant Molecular Biology (S.W., G.A.K., J.-Y.J., E.V., Y.P.) and Genomic Technologies Facility, Center for Integrative Genomics (S.P.), University of Lausanne, 1015 Lausanne, Switzerland;Vital-IT, Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland (S.P.); andInstitute for Crop Science and Natural Resources, Chemical Signaling, University of Bonn, 53113 Bonn, Germany (I.A., A.J.M.).
Isabel AllerDepartment for Plant Molecular Biology (S.W., G.A.K., J.-Y.J., E.V., Y.P.) and Genomic Technologies Facility, Center for Integrative Genomics (S.P.), University of Lausanne, 1015 Lausanne, Switzerland;Vital-IT, Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland (S.P.); andInstitute for Crop Science and Natural Resources, Chemical Signaling, University of Bonn, 53113 Bonn, Germany (I.A., A.J.M.).ORCID http://orcid.org/0000-0002-0501-807X
Andreas J MeyerDepartment for Plant Molecular Biology (S.W., G.A.K., J.-Y.J., E.V., Y.P.) and Genomic Technologies Facility, Center for Integrative Genomics (S.P.), University of Lausanne, 1015 Lausanne, Switzerland;Vital-IT, Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland (S.P.); andInstitute for Crop Science and Natural Resources, Chemical Signaling, University of Bonn, 53113 Bonn, Germany (I.A., A.J.M.).ORCID http://orcid.org/0000-0001-8144-4364
Yves PoirierDepartment for Plant Molecular Biology (S.W., G.A.K., J.-Y.J., E.V., Y.P.) and Genomic Technologies Facility, Center for Integrative Genomics (S.P.), University of Lausanne, 1015 Lausanne, Switzerland;Vital-IT, Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland (S.P.); andInstitute for Crop Science and Natural Resources, Chemical Signaling, University of Bonn, 53113 Bonn, Germany (I.A., A.J.M.) yves.poirier@unil.ch.ORCID http://orcid.org/0000-0001-8660-294X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The response of shoots to phosphate (Pi) deficiency implicates long-distance communication between roots and shoots, but the participating components are poorly understood. We have studied the topology of the Arabidopsis (Arabidopsis thaliana) PHOSPHATE1 (PHO1) Pi exporter and defined the functions of its different domains in Pi homeostasis and signaling. The results indicate that the amino and carboxyl termini of PHO1 are both oriented toward the cytosol and that the protein spans the membrane twice in the EXS domain, resulting in a total of six transmembrane α-helices. Using transient expression in Nicotiana benthamiana leaf, we demonstrated that the EXS domain of PHO1 is essential for Pi export activity and proper localization to the Golgi and trans-Golgi network, although the EXS domain by itself cannot mediate Pi export. In contrast, removal of the amino-terminal hydrophilic SPX domain does not affect the Pi export capacity of the truncated PHO1 in N. benthamiana. While the Arabidopsis pho1 mutant has low shoot Pi and shows all the hallmarks associated with Pi deficiency, including poor shoot growth and overexpression of numerous Pi deficiency-responsive genes, expression of only the EXS domain of PHO1 in the roots of the pho1 mutant results in a remarkable improvement of shoot growth despite low shoot Pi. Transcriptomic analysis of pho1 expressing the EXS domain indicates an attenuation of the Pi signaling cascade and the up-regulation of genes involved in cell wall synthesis and the synthesis or response to several phytohormones in leaves as well as an altered expression of genes responsive to abscisic acid in roots.

Indexed as

ArabidopsisArabidopsis ProteinsCytosolGene Expression Regulation, PlantNicotianaPhosphatesPlant RootsPlants, Genetically ModifiedPlant ShootsProtein Structure, TertiarySignal Transductiontrans-Golgi NetworkArabidopsis ProteinsPHO1 protein, ArabidopsisPhosphates

Identifiers

PMID26546667
PMCPMC4704572

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.