ArticleMethods (San Diego, Calif.)2016
Using in vivo imaging to measure RNA mobility in Xenopus laevis oocytes.
Article in Methods (San Diego, Calif.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Intrinsically disordered regions and RNA binding domains contribute to protein enrichment in biomolecular condensates in Xenopus oocytes.Scientific reports · 2024Article
- Intrinsically disordered regions and RNA binding domains contribute to protein enrichment in biomolecular condensates inbioRxiv : the preprint server for biology · 2023Article
- Cell Rover-a miniaturized magnetostrictive antenna for wireless operation inside living cells.Nature communications · 2022Article
- Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates iniScience · 2022Article
- Role of Zinc (Zn) in Human Reproduction: A Journey from Initial Spermatogenesis to Childbirth.International journal of molecular sciences · 2021Review
- Fluorescence techniques in developmental biology.Journal of biosciences · 2018Review
- Modeling microtubule-based transport and anchoring of mRNA.SIAM journal on applied dynamical systems · 2018Article
- Analysis of Active Transport by Fluorescence Recovery after Photobleaching.Biophysical journal · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
RNA localization in the Xenopus oocyte is responsible for the establishment of polarity during oogenesis as well as the specification of germ layers during embryogenesis. However, the inability to monitor mRNA localization in live vertebrate oocytes has posed a major barrier to understanding the mechanisms driving directional transport. Here we describe a method for imaging MS2 tagged RNA in live Xenopus oocytes to study the dynamics of RNA localization. We also focus on methods for implementing and analyzing FRAP data. This protocol is optimized for imaging of the RNAs in stage II oocytes but it can be adapted to study dynamics of other molecules during oogenesis. Using this approach, mobility can be measured in different regions of the oocyte, enabling the direct observation of molecular dynamics throughout the oocyte.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.