ArticleGenes & development2015
Transcription and imprinting dynamics in developing postnatal male germline stem cells.
Article in Genes & development, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed.
- ZBTB16/PLZF regulates juvenile spermatogonial stem cell development through an extensive transcription factor poising network.Nature structural & molecular biology · 2025Article
- Paternal Exposure to Methylphenidate Induces Poor-Quality Blastocyst and Epigenetic Changes.Molecular reproduction and development · 2025Article
- Article
- Profiling Histone Modifications in Differentiating Mouse Spermatogonia with CUT&Tag.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Quantitative CUT&Tag for Epigenomic Profiling of Mouse Germ Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The Biology and Regulation of Spermatogonial Stem Cells in the Niche.Advances in experimental medicine and biology · 2025Review
- Review
- Epigenetic priming in the male germline.Current opinion in genetics & development · 2024Review
- PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiation.Nucleic acids research · 2024Article
- The single-cell chromatin accessibility landscape in mouse perinatal testis development.eLife · 2023Article
- Chromatin Remodeling via Retinoic Acid Action during Murine Spermatogonial Development.Life (Basel, Switzerland) · 2023Article
- The homeodomain of Oct4 is a dimeric binder of methylated CpG elements.Nucleic acids research · 2023Article
- Transcriptome Profiling of Developing Testes and First Wave of Spermatogenesis in the Rat.Genes · 2023Article
- Defining Gene Function in Spermatogonial Stem Cells Through Conditional Knockout Approaches.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Decoding the Spermatogenesis Program: New Insights from Transcriptomic Analyses.Annual review of genetics · 2022Review
- LIN28 Family in Testis: Control of Cell Renewal, Maturation, Fertility and Aging.International journal of molecular sciences · 2022Review
- Mouse Chd4-NURD is required for neonatal spermatogonia survival and normal gonad development.Epigenetics & chromatin · 2022Article
- Using Experimental Models to Decipher the Effects of Acetaminophen and NSAIDs on Reproductive Development and Health.Frontiers in toxicology · 2022Review
- Cell-fate transition and determination analysis of mouse male germ cells throughout development.Nature communications · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
Postnatal spermatogonial stem cells (SSCs) progress through proliferative and developmental stages to populate the testicular niche prior to productive spermatogenesis. To better understand, we conducted extensive genomic profiling at multiple postnatal stages on subpopulations enriched for particular markers (THY1, KIT, OCT4, ID4, or GFRa1). Overall, our profiles suggest three broad populations of spermatogonia in juveniles: (1) epithelial-like spermatogonia (THY1(+); high OCT4, ID4, and GFRa1), (2) more abundant mesenchymal-like spermatogonia (THY1(+); moderate OCT4 and ID4; high mesenchymal markers), and (3) (in older juveniles) abundant spermatogonia committing to gametogenesis (high KIT(+)). Epithelial-like spermatogonia displayed the expected imprinting patterns, but, surprisingly, mesenchymal-like spermatogonia lacked imprinting specifically at paternally imprinted loci but fully restored imprinting prior to puberty. Furthermore, mesenchymal-like spermatogonia also displayed developmentally linked DNA demethylation at meiotic genes and also at certain monoallelic neural genes (e.g., protocadherins and olfactory receptors). We also reveal novel candidate receptor-ligand networks involving SSCs and the developing niche. Taken together, neonates/juveniles contain heterogeneous epithelial-like or mesenchymal-like spermatogonial populations, with the latter displaying extensive DNA methylation/chromatin dynamics. We speculate that this plasticity helps SSCs proliferate and migrate within the developing seminiferous tubule, with proper niche interaction and membrane attachment reverting mesenchymal-like spermatogonial subtype cells back to an epithelial-like state with normal imprinting profiles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.