ArticleMolecular & cellular proteomics : MCP2016
Identification of Evening Complex Associated Proteins in Arabidopsis by Affinity Purification and Mass Spectrometry.
Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
112 citing papers in PubMed, 212 citations in OpenAlex.
- Unveiling previously undescribed circadian clock regulators and action mechanisms via TurboID-based profiling of the LWD1 interactome inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Mineral nutrients as regulators of plant flowering time: A molecular perspective.Journal of integrative plant biology · 2026Review
- Integrated phylogenomic and transcriptomic analyses reveal GhTZP3 as a key gene regulating flowering time and undergoing artificial selection in upland cotton (Gossypium hirsutum).Functional & integrative genomics · 2026Article
- Phytochrome B sets condensate number through graded nucleator states and seeding-site efficacy.Nature communications · 2026Article
- Twenty-five years of photobodies: formation, composition, and the 2-compartment logic of phytochrome B signaling.Plant physiology · 2026Review
- Remote Sensing of Endogenous Pigmentation by Inducible Synthetic Circuits in Grasses.Plant biotechnology journal · 2026Article
- A genetic screen identifies the E3 ubiquitin ligase MPSR1 as a suppressor of early flowering of the sensitivity to red light reduced 1 mutant.Scientific reports · 2025Article
- The bZIP3-AS1 complex promotes CONSTANS-induced FLOWERING LOCUS T activation in a daylength-dependent manner.Journal of integrative plant biology · 2025Article
- TANDEM ZINC-FINGER/PLUS3 integrates light signaling and flowering regulatory pathways at the chromatin level.The New phytologist · 2025Article
- A multisensor high-temperature signaling framework for triggering daytime thermomorphogenesis in Arabidopsis.Nature communications · 2025Article
- Complex Signaling Networks Underlying Blue-Light-Mediated Floral Transition in Plants.Plants (Basel, Switzerland) · 2025Review
- Abundant clock proteins point to missing molecular regulation in the plant circadian clock.Molecular systems biology · 2025Article
- Integrating temperature into the Arabidopsis circadian system.Npj biological timing and sleep · 2025Review
- Breeding potential of Spanish bread wheat landraces: genetic variability in vernalization and photoperiod sensitivity.Frontiers in plant science · 2025Article
- The evening complex component ELF3 recruits H3K4me3 demethylases to repress PHYTOCHROME INTERACTING FACTOR4 and 5 in Arabidopsis.The Plant cell · 2024Article
- An eight-founder wheat MAGIC population allows fine-mapping of flowering time loci and provides novel insights into the genetic control of flowering time.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- A circadian clock output functions independently of phyB to sustain daytime PIF3 degradation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- What is going on inside of phytochrome B photobodies?The Plant cell · 2024Review
- The Function, Regulation, and Mechanism of Protein Turnover in Circadian Systems inInternational journal of molecular sciences · 2024Review
- Plants distinguish different photoperiods to independently control seasonal flowering and growth.Science (New York, N.Y.) · 2024Article
52 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Many species possess an endogenous circadian clock to synchronize internal physiology with an oscillating external environment. In plants, the circadian clock coordinates growth, metabolism and development over daily and seasonal time scales. Many proteins in the circadian network form oscillating complexes that temporally regulate myriad processes, including signal transduction, transcription, protein degradation and post-translational modification. In Arabidopsis thaliana, a tripartite complex composed of EARLY FLOWERING 4 (ELF4), EARLY FLOWERING 3 (ELF3), and LUX ARRHYTHMO (LUX), named the evening complex, modulates daily rhythms in gene expression and growth through transcriptional regulation. However, little is known about the physical interactions that connect the circadian system to other pathways. We used affinity purification and mass spectrometry (AP-MS) methods to identify proteins that associate with the evening complex in A. thaliana. New connections within the circadian network as well as to light signaling pathways were identified, including linkages between the evening complex, TIMING OF CAB EXPRESSION1 (TOC1), TIME FOR COFFEE (TIC), all phytochromes and TANDEM ZINC KNUCKLE/PLUS3 (TZP). Coupling genetic mutation with affinity purifications tested the roles of phytochrome B (phyB), EARLY FLOWERING 4, and EARLY FLOWERING 3 as nodes connecting the evening complex to clock and light signaling pathways. These experiments establish a hierarchical association between pathways and indicate direct and indirect interactions. Specifically, the results suggested that EARLY FLOWERING 3 and phytochrome B act as hubs connecting the clock and red light signaling pathways. Finally, we characterized a clade of associated nuclear kinases that regulate circadian rhythms, growth, and flowering in A. thaliana. Coupling mass spectrometry and genetics is a powerful method to rapidly and directly identify novel components and connections within and between complex signaling pathways.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.