Evidence map›Paper›PMID 26545361›Full record

ArticleHernia : the journal of hernias and abdominal wall surgery2016

Living scaffolds: surgical repair using scaffolds seeded with human adipose-derived stem cells.

A Klinger, M Kawata, M Villalobos, R B Jones, S Pike, N Wu, S Chang, P Zhang, P DiMuzio, J Vernengo and 6 more

Registry-linked trialAbstract readEvaluation Study
PubMed Publisher
In one paragraph

Article in Hernia : the journal of hernias and abdominal wall surgery, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03548610 (Efficacy and Safety of a Nanofat-seeded Biological Scaffold in Healing Lower Limb Surgical Defects), which is not on this map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
3.8field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03548610 nawithdrawnnot on this mapstarted 2019, after this paper: background citation

Efficacy and Safety of a Nanofat-seeded Biological Scaffold in Healing Lower Limb Surgical Defects: A Randomized, Controlled Study

TypeinterventionalSponsorBrigham and Women's HospitalRan2019 to 2021Enrolled0ConditionsWound of Skin, Non-melanoma Skin Cancer, Skin Graft Complications, Wound of Lower LegArmsNanofat-seeded biological scaffold on surgical defect
3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Review
  3. Therapeutic Potential of Adipose Stem Cells.Advances in experimental medicine and biology · 2021
    Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Burst inflation test for measuring biomechanical properties of rat abdominal walls.Hernia : the journal of hernias and abdominal wall surgery · 2017
    Article
  12. Review
  13. Article
  14. Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 1 country.

A KlingerDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
M KawataDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
M VillalobosDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
R B JonesDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
S PikeDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
N WuDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
S ChangDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
P ZhangDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
P DiMuzioDepartment of Surgery, Thomas Jefferson University, 1020 Walnut Street, Philadelphia, PA, 19107, USA.
J VernengoDepartment of Chemical Engineering, Rowan University, 201 Mullica Hill Road, Glassboro, NJ, 08028, USA.
P BenvenutoDepartment of Chemical Engineering, Rowan University, 201 Mullica Hill Road, Glassboro, NJ, 08028, USA.
R D GoldfarbDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
K HunterDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
Y LiuDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
J P CarpenterDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA.
T N TulenkoDepartment of Surgery, Cooper University Hospital and Cooper Medical School at Rowan University, 3 Cooper Plaza, Camden, NJ, 08108, USA. tulenko-thomas@cooperhealth.edu.
Cooper University Hospital · USRowan University · USThomas Jefferson University · US

Funding

Dietary Cholesterol and Defects in Cholesterol SynthesisR01HL073980 · NHLBI · OREGON HEALTH & SCIENCE UNIVERSITY · PI STEINER, ROBERT DAVID · 2003 to 2013
$4.5M
NHLBI NIH HHS R01HL073980
6 · The paper itself

Abstract

backgroundDecellularized porcine small intestinal submucosa (SIS) is a biological scaffold used surgically for tissue repair. Here, we demonstrate a model of SIS as a scaffold for human adipose-derived stem cells (ASCs) in vitro and apply it in vivo in a rat ventral hernia repair model. STUDY

designASCs adherence was examined by confocal microscopy and proliferation rate was measured by growth curves. Multipotency of ASCs seeded onto SIS was tested using adipogenic, chondrogenic, and osteogenic induction media. For in vivo testing, midline abdominal musculofascial and peritoneal defects were created in Sprague-Dawley rats. Samples were evaluated for tensile strength, histopathology and immunohistochemistry.

resultsAll test groups showed cell adherence and proliferation on SIS. Fibronectin-treated scaffolds retained more cells than those treated with vehicle alone (p < 0.05). Fresh stromal vascular fraction (SVF) pellets containing ASCs were injected onto the SIS scaffold and showed similar results to cultured ASCs. Maintenance of multipotency on SIS was confirmed by lineage-specific markers and dyes. Histopathology revealed neovascularization and cell influx to ASC-seeded SIS samples following animal implantation. ASC-seeded SIS appeared to offer a stronger repair than plain SIS, but these results were not statistically significant. Immunohistochemistry showed continued presence of cells of human origin in ASC-seeded repairs at 1 month postoperation.

conclusionPretreatment of the scaffold with fibronectin offers a method to increase cell adhesion and delivery. ASCs maintain their immunophenotype and ability to differentiate while on SIS. Seeding freshly isolated SVF onto the scaffold demonstrated that minimally manipulated cells may be useful for perioperative surgical applications within the OR suite. We have shown that this model for a "living mesh" can be successfully used in abdominal wall reconstruction.

Indexed as

Tissue ScaffoldsAnimalsCell ProliferationFemaleHernia, VentralHerniorrhaphyHumansIntestinal MucosaIntestine, SmallMesenchymal Stem CellsMicroscopy, ConfocalModels, AnimalRatsRats, Sprague-DawleySwineTensile StrengthHerniaMesenchymal stem cellsSISSmall intestine submucosa

Identifiers

PMID26545361
OpenAlexW2135228592

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.