ArticleAngewandte Chemie (International ed. in English)2015
In-Membrane Chemical Modification (IMCM) for Site-Specific Chromophore Labeling of GPCRs.
Article in Angewandte Chemie (International ed. in English), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
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- Membrane mimetic-dependence of GPCR energy landscapes.Structure (London, England : 1993) · 2024Article
- Membrane Mimetic-Dependence of GPCR Energy Landscapes.bioRxiv : the preprint server for biology · 2023Article
- Dual mechanisms of cholesterol-GPCR interactions that depend on membrane phospholipid composition.Structure (London, England : 1993) · 2023Article
- Selective polypeptide ligand binding to the extracellular surface of the transmembrane domains of the class B GPCRs GLP-1R and GCGR.iScience · 2023Article
- Bioorthogonal Tethering Enhances Drug Fragment Affinity for G Protein-Coupled Receptors in Live Cells.Journal of the American Chemical Society · 2023Article
- Sub-millisecond conformational dynamics of the ACommunications biology · 2023Article
- Anionic phospholipids control mechanisms of GPCR-G protein recognition.Nature communications · 2023Article
- Anionic Phospholipids Control Mechanisms of GPCR-G Protein Recognition.bioRxiv : the preprint server for biology · 2023Article
- A method for selectiveProtein science : a publication of the Protein Society · 2022Article
- GPCR structural characterization by NMR spectroscopy in solution.Acta biochimica et biophysica Sinica · 2022Review
- G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling.Molecules (Basel, Switzerland) · 2022Review
- Production of a Human Histamine Receptor for NMR Spectroscopy in Aqueous Solutions.Biomolecules · 2021Article
- GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures.Nature reviews. Drug discovery · 2019Review
- AProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Extrinsic Tryptophans as NMR Probes of Allosteric Coupling in Membrane Proteins: Application to the AJournal of the American Chemical Society · 2018Article
- Deuteration and selective labeling of alanine methyl groups of βJournal of biomolecular NMR · 2018Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
We present in-membrane chemical modification (IMCM) for obtaining selective chromophore labeling of intracellular surface cysteines in G-protein-coupled receptors (GPCRs) with minimal mutagenesis. This method takes advantage of the natural protection of most cysteines by the membrane environment. Practical use of IMCM is illustrated with the site-specific introduction of chromophores for NMR and fluorescence spectroscopy in the human κ-opioid receptor (KOR) and the human A2A adenosine receptor (A2A AR). IMCM is applicable to a wide range of in vitro studies of GPCRs, including single-molecule spectroscopy, and is a promising platform for in-cell spectroscopy experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.