ArticlePloS one2015
Killing of Mycolic Acid-Containing Bacteria Aborted Induction of Antibiotic Production by Streptomyces in Combined-Culture.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- A phage-derived reconfigurable effector associated with an actinobacterial contractile nanomachine tailors bacterial responses to competition.Journal of bacteriology · 2026Article
- Expanding catalytic versatility of modular polyketide synthases for alcohol biosynthesis.Nature chemical biology · 2025Article
- Griseolutein T from Streptomyces seoulensis, newly identified via combined-culture with Tsukamurella pulmonis, as an efficacious therapeutic agent against multidrug-resistant bacteria.The Journal of antibiotics · 2025Article
- Co-cultivation strategies for natural product discovery from actinomycetes: unlocking silent secondary metabolism with mycolic acid-containing bacteria.World journal of microbiology & biotechnology · 2025Review
- Aspergillus terreus IFM 65899-THP-1 cells interaction triggers production of the natural product butyrolactone Ia, an immune suppressive compound.Scientific reports · 2024Article
- Methodology for awakening the potential secondary metabolic capacity in actinomycetes.Beilstein journal of organic chemistry · 2024Review
- Transcriptionally induced nucleoid-associated protein-likeFrontiers in microbiology · 2024Article
- Interactions of DifferentInternational journal of molecular sciences · 2023Article
- Bioactive Metabolites from Terrestrial and Marine Actinomycetes.Molecules (Basel, Switzerland) · 2023Review
- Regulation of Multidrug Efflux Pumps by TetR Family Transcriptional Repressor Negatively Affects Secondary Metabolism in Streptomyces coelicolor A3(2).Applied and environmental microbiology · 2023Article
- Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production inAntibiotics (Basel, Switzerland) · 2022Article
- Intimate relationships among actinomycetes and mycolic acid-containing bacteria.Scientific reports · 2022Article
- Article
- Activation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria.Microorganisms · 2021Article
- Phage tail-like nanostructures affect microbial interactions between Streptomyces and fungi.Scientific reports · 2021Article
- Discovery of "heat shock metabolites" produced by thermotolerant actinomycetes in high-temperature culture.The Journal of antibiotics · 2020Article
- Microbe-driven chemical ecology: past, present and future.The ISME journal · 2019Review
- Activation of silent biosynthetic pathways and discovery of novel secondary metabolites in actinomycetes by co-culture with mycolic acid-containing bacteria.Journal of industrial microbiology & biotechnology · 2019Review
- Symbiosis-inspired approaches to antibiotic discovery.Natural product reports · 2017Review
- Novel antibiotic screening methods to awaken silent or cryptic secondary metabolic pathways in actinomycetes.The Journal of antibiotics · 2017Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Co-culture of Streptomyces with mycolic acid-containing bacteria (MACB), which we termed "combined-culture," alters the secondary metabolism pattern in Streptomyces and has been a useful method for the discovery of bioactive natural products. In the course of our investigation to identify the inducing factor(s) of MACB, we previously observed that production of pigments in Streptomyces lividans was not induced by factors such as culture extracts or mycolic acids. Although dynamic changes occurred in culture conditions because of MACB, the activation of pigment production by S. lividans was observed in a limited area where both colonies were in direct contact. This suggested that direct attachment of cells is a requirement and that components on the MACB cell membrane may play an important role in the response by S. lividans. Here we examined whether this response was influenced by dead MACB that possess intact mycolic acids assembled on the outer cell membrane. Formaldehyde fixation and γ-irradiation were used to prepare dead cells that retain their shape and mycolic acids of three MACB species: Tsukamurella pulmonis, Rhodococcus erythropolis, and Rhodococcus opacus. Culturing tests verified that S. lividans does not respond to the intact dead cells of three MACB. Observation of combined-culture by scanning electron microscopy (SEM) indicated that adhesion of live MACB to S. lividans mycelia were a significant interaction that resulted in formation of co-aggregation. In contrast, in the SEM analysis, dead cells were not observed to adhere. Therefore, direct attachment by live MACB cells is proposed as one of the possible factors that causes Streptomyces to alter its specialized metabolism in combined-culture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.