Evidence map›Paper›PMID 26543340›Full record

ArticleChinese journal of cancer research = Chung-kuo yen cheng yen chiu2015

A PTEN translational isoform has PTEN-like activity.

Xie Zhang, Bowei Yin, Fangfang Zhu, Guochang Huang, Hong Li

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Xie Zhang1 Department of Gastroenterology, Ningbo Medical Center, LiHuiLi Hospital, Ningbo 315040, Zhejiang, China ; 2 School of Medicine, Ningbo University, Ningbo 315211, Zhejiang, China ; 3 Ningbo Medical Center, 4 Minimally Invasive Abdominal Surgery, Ningbo Medical Center, LiHuiLi Hospital, Ningbo 315040, Zhejiang Province, China.
Bowei Yin1 Department of Gastroenterology, Ningbo Medical Center, LiHuiLi Hospital, Ningbo 315040, Zhejiang, China ; 2 School of Medicine, Ningbo University, Ningbo 315211, Zhejiang, China ; 3 Ningbo Medical Center, 4 Minimally Invasive Abdominal Surgery, Ningbo Medical Center, LiHuiLi Hospital, Ningbo 315040, Zhejiang Province, China.
Fangfang Zhu1 Department of Gastroenterology, Ningbo Medical Center, LiHuiLi Hospital, Ningbo 315040, Zhejiang, China ; 2 School of Medicine, Ningbo University, Ningbo 315211, Zhejiang, China ; 3 Ningbo Medical Center, 4 Minimally Invasive Abdominal Surgery, Ningbo Medical Center, LiHuiLi Hospital, Ningbo 315040, Zhejiang Province, China.
Guochang Huang1 Department of Gastroenterology, Ningbo Medical Center, LiHuiLi Hospital, Ningbo 315040, Zhejiang, China ; 2 School of Medicine, Ningbo University, Ningbo 315211, Zhejiang, China ; 3 Ningbo Medical Center, 4 Minimally Invasive Abdominal Surgery, Ningbo Medical Center, LiHuiLi Hospital, Ningbo 315040, Zhejiang Province, China.
Hong Li1 Department of Gastroenterology, Ningbo Medical Center, LiHuiLi Hospital, Ningbo 315040, Zhejiang, China ; 2 School of Medicine, Ningbo University, Ningbo 315211, Zhejiang, China ; 3 Ningbo Medical Center, 4 Minimally Invasive Abdominal Surgery, Ningbo Medical Center, LiHuiLi Hospital, Ningbo 315040, Zhejiang Province, China.
Ningbo Medical Center Lihuili Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTo identify PTEN isoform and explore its potential role in tumor suppression.

methodsWestern blotting, over-expression, shRNA mediated knocking-down, and bioinformatic analysis were used to identify PTEN isoform and test its effect on PI3K-Akt signaling pathway. Cell proliferation, apoptosis, and migration assays were used to test PTEN isoform's biological activities.

resultsThe PTEN isoform is about 15 kDa bigger than PTEN and its expression is dependent on PTEN status. Immunoprecipitation for PTEN isoform followed by screening with antibodies against ISG15, SUMO1/2/3, Ubiquitin, and Nedd8 showed the identified PTEN isoform is not a general proteinaceous post-translational modification. In addition, overexpression of PTEN cDNA in cells did not generate PTEN isoform whereas knocking-down of PTEN reduced the protein levels of both PTEN and PTEN isoform in a proportional manner. Analysis of PTEN DNA sequence disclosed an alternative translational starting code (CTG) upstream of canonical PTEN coding sequence. Expression of cloned PTEN isoform generated a protein with a size about 15 kDa bigger than PTEN and suppressed PI3K-Akt signaling pathway in cells. Overexpression of PTEN isoform also led to decrease in cell growth and enhanced serum starvation-and UV irradiation-induced apoptosis through activation of Caspase 3. Finally, expression of PTEN isoform inhibited cell migration in scratch assay.

conclusionsPTEN isoform has PTEN-like activity and might be a new tumor suppressor.

Indexed as

apoptosiscell growthcell migrationPI3K-AktPTENPTEN isoformtumor suppressor

Identifiers

PMID26543340
PMCPMC4626823
OpenAlexW2128135207

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.