ArticleIndian journal of microbiology2015
Improvement in Oil Production by Increasing Malonyl-CoA and Glycerol-3-Phosphate Pools in Scenedesmus quadricauda.
Article in Indian journal of microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 63 citations in OpenAlex.
- Next-generation biodiesel production: catalytic innovations and scalable microalgal biorefinery systems.RSC advances · 2026Review
- Metabolic Engineering of Microalgae for Biofuel Production.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Two strategies to improve the supply of PKS extender units for ansamitocin P-3 biosynthesis by CRISPR-Cas9.Bioresources and bioprocessing · 2022Article
- Production of microalgae with high lipid content and their potential as sources of nutraceuticals.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2022Review
- Enhancing microalgal lipid accumulation for biofuel production.Frontiers in microbiology · 2022Review
- Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture.Molecules (Basel, Switzerland) · 2021Review
- A High Temperature Environment Regulates the Olive Oil Biosynthesis Network.Plants (Basel, Switzerland) · 2020Article
- Genomic insights into the lifestyles, functional capacities and oleagenicity of members of the fungal family Trichosporonaceae.Scientific reports · 2020Article
- Exploring the Role of a Cytokinin-Activating Enzyme LONELY GUY in Unicellular MicroalgaFrontiers in plant science · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, microalgae have attracted considerable interest as a biofuel resource owing to their rapid growth, tolerance to harsh conditions, and ability to accumulate a large amount of triacylglycerols (TAGs). However, the economic effectiveness of algal biofuel is still low. In this study, we attempted to increase oil production of the microalga Scenedesmus quadricauda by elevating intracellular malonyl-CoA and glycerol-3-phosphate (G3P) pools. To increase intracellular oil content, yeast-derived genes encoding acetyl-CoA carboxylase (ACC1), glycerol kinase (GPD1), and glycerol-3-phosphate dehydrogenase (GUT1) were overexpressed under the control of CaMV 35S and NOS promoters with SV40 large T antigen components. Fatty acid profiling, G3P content, and the number of cells with high oil content were analyzed by gas chromatography-mass spectrometry, G3P assay kit, and flow cytometry, respectively. Overexpression of ACC1 increased the total fatty acid content by 1.6-fold. Overexpression of GPD1 and GUT1 increased intracellular G3P content by 1.6- and 1.9-fold, respectively. Multi-gene expression of ACC1, GPD1, and GUT1 increased the number of cells with high oil content by 1.45-fold compared with that observed with the wild-type. This study is the first to report increased oil production by overexpression of the key genes (ACC1, GPD1, and GUT1) for TAG biosynthesis in microalgae.
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