ArticleJournal of ovarian research2015
Ovarian stem cells are always accompanied by very small embryonic-like stem cells in adult mammalian ovary.
Article in Journal of ovarian research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- In Vitro Gamete Production in Mammals: Decades of Pioneering Approaches in Germ Cell and Stem Cell Biology Supporting Innovative Discovery and Expansion of Human Reproductive Potential.Molecular reproduction and development · 2025Review
- Small adipose-derived mesenchymal stromal cells exhibit longer telomeres and enhanced regenerative potential.Frontiers in bioengineering and biotechnology · 2025Article
- Oogonial stem cells: the unexpected superheroes.Reproduction & fertility · 2024Article
- Review
- Correction to: ovarian stem cells are always accompanied by very small embryonic-like stem cells in adult mammalian ovary.Journal of ovarian research · 2023Article
- Isolation of Swine Bone Marrow Lin-/CD45-/CD133 + Cells and Cardio-protective Effects of its Exosomes.Stem cell reviews and reports · 2023Article
- Inhibition of EED activity enhances cell survival of female germline stem cell and improves the oocytes production during oogenesisOpen biology · 2023Article
- Germline stem cells in human.Signal transduction and targeted therapy · 2022Review
- Female germline stem cells: aging and anti-aging.Journal of ovarian research · 2022Review
- Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro.Histochemistry and cell biology · 2021Article
- In Vitro Generation of Oocyte Like Cells and Their In Vivo Efficacy: How Far We have been Succeeded.Cells · 2020Review
- In Vitro Generation of Oocytes from Ovarian Stem Cells (OSCs): In Search of Major Evidence.International journal of molecular sciences · 2019Review
- Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness.Stem cell research & therapy · 2019Article
- The ovarian germinal reserve and apoptosis-related proteins in the infant and adolescent human ovary.Journal of ovarian research · 2019Article
- Stem Cells and Progenitors in Human Peripheral Blood Get Activated by Extremely Active Resveratrol (XAR™).Stem cell reviews and reports · 2018Article
- Ovarian stem cells-resolving controversies.Journal of assisted reproduction and genetics · 2018Article
- Further characterization of adult sheep ovarian stem cells and their involvement in neo-oogenesis and follicle assembly.Journal of ovarian research · 2018Article
- Making gametes from alternate sources of stem cells: past, present and future.Reproductive biology and endocrinology : RB&E · 2017Review
- Adult human pancreas-derived cells expressing stage-specific embryonic antigen 4 differentiate into Sox9-expressing and Ngn3-expressing pancreatic ducts in vivo.Stem cell research & therapy · 2016Article
- Overexpression of Oct4 in porcine ovarian stem/stromal cells enhances differentiation of oocyte-like cells in vitro and ovarian follicular formation in vivo.Journal of ovarian research · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExisting dogma that a female is born with fixed number of eggs was challenged by the detection of stem cells in adult mammalian ovary. Data has accumulated in support of ovarian stem cells (OSCs) proliferation, maintenance in culture, formation of germ cell nests and differentiation into oocytes and primordial follicle assembly using different strategies.
resultsFlow cytometry analysis identified >8 μm OSCs which are DDX1 positive and are considered equivalent to spermatogonial stem cells (SSCs) in testis. Analysis of both ovarian and testicular smears obtained after enzymatic digestion has led to the identification of an additional stem cell population termed very small embryonic-like stem cells (VSELs). VSELs and OSCs/SSCs differ from each other in their size and OCT-4 expression. VSELs express pluripotent markers including nuclear OCT-4 whereas OSCs/SSCs express cytoplasmic OCT-4 suggesting a differentiated state. VSELs can be studied by flow cytometry as small sized cells which are LIN-/CD45-/Sca-1+. We have reported 0.02 ± 0.008, 0.03 ± 0.017 and 0.08 ± 0.03 % of total cells as VSELs in normal, chemoablated and after FSH treatment to chemoablated mouse ovary.
conclusionsVSELs have remained poorly studied till now because of their very small size and rare occurrence. Spinning cells obtained after enzymatic digestion of ovarian tissue at a speed of 1000G (rather than 1200 rpm) throughout processing allows reliable detection of the VSELs by flow cytometry. VSELs exist in aged, chemoablated and non-functional ovary and providing a healthy niche to support their function offers an interesting strategy to manage infertility.
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