ArticleMicrobial cell factories2015
Significantly improved solvent tolerance of Escherichia coli by global transcription machinery engineering.
Article in Microbial cell factories, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Reduced efficacy of food-chain biocides and preservatives against ciprofloxacin-resistant Salmonella Typhimurium.Applied microbiology and biotechnology · 2026Article
- Adaptive cargo deletion as an evolutionary dead-end during phage-based directed evolution.BMC research notes · 2026Article
- Heterologous expression of a heme-dependent catalase from Avicennia marina confers multi-stress tolerance in Escherichia coli for biotechnological applications.Scientific reports · 2026Article
- Genetic "expiry-date" circuits control lifespan of synthetic scavenger bacteria for safe bioremediation.Nucleic acids research · 2025Article
- Enhanced chaotrope tolerance and (S)-2-hydroxypropiophenone production by recombinant Pseudomonas putida engineered with Pprl from Deinococcus radiodurans.Microbial biotechnology · 2024Article
- Advance of tolerance engineering on microbes for industrial production.Synthetic and systems biotechnology · 2023Review
- Improving prodigiosin production by transcription factor engineering and promoter engineering inFrontiers in microbiology · 2022Article
- A cold shock protein promotes high-temperature microbial growth through binding to diverse RNA species.Cell discovery · 2021Article
- Rapid Enabling ofFrontiers in bioengineering and biotechnology · 2021Article
- Escherichia coli adaptation and response to exposure to heavy atmospheric pollution.Scientific reports · 2019Article
- Article
- Enhancing butanol tolerance ofBiotechnology for biofuels · 2019Article
- Synthetic biology for evolutionary engineering: from perturbation of genotype to acquisition of desired phenotype.Biotechnology for biofuels · 2019Review
- Identification and manipulation of a novel locus to improve cell tolerance to short-chain alcohols in Escherichia coli.Journal of industrial microbiology & biotechnology · 2018Article
- Improved xylose tolerance and 2,3-butanediol production ofBiotechnology for biofuels · 2018Article
- Improving phloroglucinol tolerance and production in Escherichia coli by GroESL overexpression.Microbial cell factories · 2017Article
- Assignment of sigma factors of RNA polymerase to promoters in Corynebacterium glutamicum.AMB Express · 2017Article
- DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance in Escherichia coli.Biotechnology for biofuels · 2016Article
- High-Throughput Method for Automated Colony and Cell Counting by Digital Image Analysis Based on Edge Detection.PloS one · 2016Article
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6 authors.
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Abstract
backgroundEscherichia coli has emerged as a promising platform microorganism to produce biofuels and fine chemicals of industrial interests. Certain obstacles however remain to be overcome, among which organic-solvent tolerance is a crucial one.
resultsWe used global transcription machinery engineering (gTME) to improve the organic-solvent tolerance (OST) of E. coli JM109. A mutant library of σ(70) encoded by rpoD was screened under cyclohexane pressure. E. coli JM109 strain harboring σ(70) mutant C9 was identified with capability of tolerating 69 % cyclohexane. The rpoD mutant contains three amino-acid substitutes and a stop-codon mutation, resulting a truncated sequence containing regions σ(1.1) and σ(1.2). Total protein difference produced by E. coli JM109 strain harboring C9 was examined with 2D-PAGE, and 204 high-abundant proteins showed over twofold variation under different solvent stress.
conclusionsOur results show that several genes (gapA, sdhB, pepB and dppA) play critical roles in enhanced solvent tolerance of E. coli, mainly involving in maintaining higher intracellular energy level under solvent stress. Global transcription machinery engineering is therefore a feasible and efficient approach for engineering strain with enhanced OST-phenotype.
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