ArticleScientific reports2015
A High-content screen identifies compounds promoting the neuronal differentiation and the midbrain dopamine neuron specification of human neural progenitor cells.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 26 citations in OpenAlex.
- Neuro-Transcriptomic Responses to Polypharmacological Agents inBrain sciences · 2026Article
- Pluripotent Stem Cell-derived Dopaminergic Neurons for Studying Developmental Neurotoxicity.Stem cell reviews and reports · 2023Review
- Multi-target regulatory mechanism of Yang Xin Tang - a traditional Chinese medicine against dementia.Chinese medicine · 2023Article
- Improved Image Analysis for Measuring Gastric Ulcer Index in Animal Models and Clinical Diagnostic Data.Diagnostics (Basel, Switzerland) · 2022Article
- Microfluidic three-dimensional cell culture of stem cells for high-throughput analysis.World journal of stem cells · 2019Review
- Small molecules re-establish neural cell fate of human fibroblasts via autophagy activation.In vitro cellular & developmental biology. Animal · 2019Article
- Identification of GSK3β inhibitor kenpaullone as a temozolomide enhancer against glioblastoma.Scientific reports · 2019Article
- A dynamic view of the proteomic landscape during differentiation of ReNcell VM cells, an immortalized human neural progenitor line.Scientific data · 2019Article
- Crosstalk of Intercellular Signaling Pathways in the Generation of Midbrain Dopaminergic Neurons In Vivo and from Stem Cells.Journal of developmental biology · 2019Review
- High-throughput identification of factors promoting neuronal differentiation of human neural progenitor cells in microscale 3D cell culture.Biotechnology and bioengineering · 2019Article
- Novel 3D Liquid Cell Culture Method for Anchorage-independent Cell Growth, Cell Imaging and Automated Drug Screening.Scientific reports · 2018Article
- High content analysis in amyotrophic lateral sclerosis.Molecular and cellular neurosciences · 2017Review
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Authors and funding
10 authors at 2 institutions in 1 country.
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Abstract
Small molecule compounds promoting the neuronal differentiation of stem/progenitor cells are of pivotal importance to regenerative medicine. We carried out a high-content screen to systematically characterize known bioactive compounds, on their effects on the neuronal differentiation and the midbrain dopamine (mDA) neuron specification of neural progenitor cells (NPCs) derived from the ventral mesencephalon of human fetal brain. Among the promoting compounds three major pharmacological classes were identified including the statins, TGF-βRI inhibitors, and GSK-3 inhibitors. The function of each class was also shown to be distinct, either to promote both the neuronal differentiation and mDA neuron specification, or selectively the latter, or promote the former but suppress the latter. We then carried out initial investigation on the possible mechanisms underlying, and demonstrated their applications on NPCs derived from human pluripotent stem cells (PSCs). Our study revealed the potential of several small molecule compounds for use in the directed differentiation of human NPCs. The screening result also provided insight into the signaling network regulating the differentiation of human NPCs.
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