ArticleGenome biology2015
A transient ischemic environment induces reversible compaction of chromatin.
Article in Genome biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 78 citations in OpenAlex.
- Proximity labeling at H3K9me3 reveals VRK-1 regulate global chromatin distribution inbioRxiv : the preprint server for biology · 2026Article
- Architecture and regulation of nanoscale chromatin domains.Nature communications · 2026Review
- Hypoxic regulation of chromatin and gene transcription.Communications biology · 2026Article
- Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications.Journal of cell science · 2025Article
- Development of a multi-targeted chemotherapeutic approach based on G-quadruplex stabilisation and carbonic anhydrase inhibition.Journal of enzyme inhibition and medicinal chemistry · 2024Article
- Hypoxia-Inducible Factor-Dependent and Independent Mechanisms Underlying Chemoresistance of Hypoxic Cancer Cells.Cancers · 2024Review
- Chromatin balances cell redox and energy homeostasis.Epigenetics & chromatin · 2023Review
- Metabolic reprogramming by immune-responsive gene 1 up-regulation improves donor heart preservation and function.Science translational medicine · 2023Article
- A comprehensive method to study the DNA's association with lamin and chromatin compaction in intact cell nuclei at super resolution.Nanoscale · 2023Article
- Single-nucleosome imaging reveals steady-state motion of interphase chromatin in living human cells.Science advances · 2022Article
- Super-resolution microscopy: a brief history and new avenues.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2022Article
- Loops, topologically associating domains, compartments, and territories are elastic and robust to dramatic nuclear volume swelling.Scientific reports · 2022Article
- Meiosis initiation: a story of two sexes in all creatures great and small.The Biochemical journal · 2021Review
- Mesenchymal Stem Cell Transplantation for Ischemic Diseases: Mechanisms and Challenges.Tissue engineering and regenerative medicine · 2021Review
- Tackling Tumour Cell Heterogeneity at the Super-Resolution Level in Human Colorectal Cancer Tissue.Cancers · 2021Article
- Non-coding RNAs in chromatin folding and nuclear organization.Cellular and molecular life sciences : CMLS · 2021Review
- The roles of inducible chromatin and transcriptional memory in cellular defense system responses to redox-active pollutants.Free radical biology & medicine · 2021Review
- Promoter G-quadruplex folding precedes transcription and is controlled by chromatin.Genome biology · 2021Article
- Selective modulation by PARP-1 of HIF-1α-recruitment to chromatin during hypoxia is required for tumor adaptation to hypoxic conditions.Redox biology · 2021Article
- Hypoxic Regulation of Gene Transcription and Chromatin: Cause and Effect.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCells detect and adapt to hypoxic and nutritional stress through immediate transcriptional, translational and metabolic responses. The environmental effects of ischemia on chromatin nanostructure were investigated using single molecule localization microscopy of DNA binding dyes and of acetylated histones, by the sensitivity of chromatin to digestion with DNAseI, and by fluorescence recovery after photobleaching (FRAP) of core and linker histones.
resultsShort-term oxygen and nutrient deprivation of the cardiomyocyte cell line HL-1 induces a previously undescribed chromatin architecture, consisting of large, chromatin-sparse voids interspersed between DNA-dense hollow helicoid structures 40-700 nm in dimension. The chromatin compaction is reversible, and upon restitution of normoxia and nutrients, chromatin transiently adopts a more open structure than in untreated cells. The compacted state of chromatin reduces transcription, while the open chromatin structure induced upon recovery provokes a transitory increase in transcription. Digestion of chromatin with DNAseI confirms that oxygen and nutrient deprivation induces compaction of chromatin. Chromatin compaction is associated with depletion of ATP and redistribution of the polyamine pool into the nucleus. FRAP demonstrates that core histones are not displaced from compacted chromatin; however, the mobility of linker histone H1 is considerably reduced, to an extent that far exceeds the difference in histone H1 mobility between heterochromatin and euchromatin.
conclusionsThese studies exemplify the dynamic capacity of chromatin architecture to physically respond to environmental conditions, directly link cellular energy status to chromatin compaction and provide insight into the effect ischemia has on the nuclear architecture of cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.