ArticleNature protocols2015
Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 198 citations in OpenAlex.
- Mapping Protein Diffusion from the Plasma Membrane to the Nucleus: Insights from Fluorescence Correlation Spectroscopy.Biomolecules · 2026Review
- Measuring morphogen transport over multiple spatial scales in live zebrafish embryos.Nature communications · 2026Article
- Spatial triple-correlation spectroscopy reveals heterotrimer dynamics in live cells.Biophysical journal · 2026Article
- Temporal variance mapping with machine learning for label-free 3D chromatin imaging using optical interferometric microscopy.Biomedical optics express · 2026Article
- Transient binding facilitates super-resolution imaging of functional amyloid fibrils on living bacteria.Protein science : a publication of the Protein Society · 2026Article
- Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A rapid method of evaluating cytotoxic drug efficacy using sub-cellular fluctuation imaging.Scientific reports · 2025Article
- Critical roles of plasma membrane dynamics and heterogeneity at the early stage of antigen-stimulated FcεRI signaling in mast cells.Biochemical Society transactions · 2025Review
- Disentangling Timescales of Molecular Kinetics with spFRET using ALEX-FCS.Journal of fluorescence · 2025Article
- Pair correlation microscopy of intracellular molecular transport.Nature protocols · 2025Review
- Statistical analysis of fluorescence intensity transients with Bayesian methods.Science advances · 2025Article
- Super-Resolution Mapping and Quantification of Molecular Diffusion via Single-Molecule Displacement/Diffusivity Mapping (SMAccounts of chemical research · 2025Article
- Cross-Correlation Increases Sampling in Diffusion-Based Super-Resolution Optical Fluctuation Imaging.Chemical & biomedical imaging · 2024Article
- Lipid-driven interleaflet coupling of plasma membrane order regulates FcεRI signaling in mast cells.Biophysical journal · 2024Article
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- Deep learning reduces data requirements and allows real-time measurements in imaging FCS.Biophysical journal · 2024Article
- Transmembrane domain-driven PD-1 dimers mediate T cell inhibition.Science immunology · 2024Article
- Wide-field intensity fluctuation imaging.Biomedical optics express · 2024Article
- Long-range formation of the Bicoid gradient requires multiple dynamic modes that spatially vary across the embryo.Development (Cambridge, England) · 2024Article
- Deubiquitination Detection of p53 Protein in Living Cells by Fluorescence Cross-Correlation Spectroscopy.ACS omega · 2023Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-plane illumination (SPIM) or total internal reflection fluorescence (TIRF) microscopes can be combined with fast and single-molecule-sensitive cameras to allow spatially resolved fluorescence (cross-) correlation spectroscopy (FCS or FCCS, hereafter referred to FCS/FCCS). This creates a powerful quantitative bioimaging tool that can generate spatially resolved mobility and interaction maps with hundreds to thousands of pixels per sample. These massively parallel imaging schemes also cause less photodamage than conventional single-point confocal microscopy-based FCS/FCCS. Here we provide guidelines for imaging FCS/FCCS measurements on commercial and custom-built microscopes (including sample preparation, setup calibration, data acquisition and evaluation), as well as anticipated results for a variety of in vitro and in vivo samples. For a skilled user of an available SPIM or TIRF setup, sample preparation, microscope alignment, data acquisition and data fitting, as described in this protocol, will take ∼1 d, depending on the sample and the mode of imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.