ArticlePloS one2015
Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Model-Guided Optimization of a Low-Serum Medium for Madin-Darby Canine Kidney Cells Using a Kolmogorov-Arnold Network and Bayesian Optimization.Applied biochemistry and biotechnology · 2026Article
- Strain Agnostic Influenza Virus Propagation in a Serum-Free, Suspension-Adapted MDCK Cell Line.Influenza and other respiratory viruses · 2026Article
- Preclinical immunogenicity and safety evaluation of MDCK cell-derived quadrivalent influenza virus subunit vaccine.Frontiers in immunology · 2026Article
- Deficiency of IFNAR1 Increases the Production of Influenza Vaccine Viruses in MDCK Cells.Viruses · 2025Article
- Development of Serum-Free Culture Systems for an Immortalized Porcine Kidney-Derived Macrophage Cell Line.Animals : an open access journal from MDPI · 2025Article
- Innovations in cell culture-based influenza vaccine manufacturing - from static cultures to high cell density cultivations.Human vaccines & immunotherapeutics · 2024Review
- From single-cell cloning to high-yield influenza virus production - implementing advanced technologies in vaccine process development.Engineering in life sciences · 2024Article
- The peculiar characteristics and advancement in diagnostic methodologies of influenza A virus.Frontiers in microbiology · 2024Review
- Apolipoprotein E mediates cell resistance to influenza virus infection.Science advances · 2022Article
- Article
- Transcriptomic Characterization Reveals Attributes of High Influenza Virus Productivity in MDCK Cells.Viruses · 2021Article
- Establishment of a Suspension MDBK Cell Line in Serum-Free Medium for Production of Bovine Alphaherpesvirus-1.Vaccines · 2021Article
- Cell-based influenza vaccine: current production,Human vaccines & immunotherapeutics · 2021Article
- Tracking changes in adaptation to suspension growth for MDCK cells: cell growth correlates with levels of metabolites, enzymes and proteins.Applied microbiology and biotechnology · 2021Article
- High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells.Applied microbiology and biotechnology · 2021Article
- Beneficial effect of Indigo Naturalis on acute lung injury induced by influenza A virus.Chinese medicine · 2020Article
- Cell Bank Origin of MDCK Parental Cells Shapes Adaptation to Serum-Free Suspension Culture and Canine Adenoviral Vector Production.International journal of molecular sciences · 2020Article
- Development of high-growth influenza H7N9 prepandemic candidate vaccine viruses in suspension MDCK cells.Journal of biomedical science · 2020Article
- Rapid process development of serum-free pseudorabies virus production with the Quality by Design approach.Cytotechnology · 2020Article
- Semliki Forest Virus replicon particles production in serum-free medium BHK-21 cell cultures and their use to express different proteins.Cytotechnology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Development of serum-free suspension cell culture processes is very important for influenza vaccine production. Previously, we developed a MDCK suspension cell line in a serum-free medium. In the present study, the growth kinetics of suspension MDCK cells and influenza virus production in the serum-free medium were investigated, in comparison with those of adherent MDCK cells in both serum-containing and serum-free medium. It was found that the serum-free medium supported the stable subculture and growth of both adherent and suspension cells. In batch culture, for both cell lines, the growth kinetics in the serum-free medium was comparable with those in the serum-containing medium and a commercialized serum-free medium. In the serum-free medium, peak viable cell density (VCD), haemagglutinin (HA) and median tissue culture infective dose (TCID50) titers of the two cell lines reached 4.51×106 cells/mL, 2.94Log10(HAU/50 μL) and 8.49Log10(virions/mL), and 5.97×106 cells/mL, 3.88Log10(HAU/50 μL), and 10.34Log10(virions/mL), respectively. While virus yield of adherent cells in the serum-free medium was similar to that in the serum-containing medium, suspension culture in the serum-free medium showed a higher virus yield than adherent cells in the serum-containing medium and suspension cells in the commercialized serum-free medium. However, the percentage of infectious viruses was lower for suspension culture in the serum-free medium. These results demonstrate the great potential of this suspension MDCK cell line in serum-free medium for influenza vaccine production and further improvements are warranted.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.