ArticleFrontiers in microbiology2015
Interactions of Dnd proteins involved in bacterial DNA phosphorothioate modification.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Molecular Insight into the Recognition of DNA by the DndCDE Complex in DNA Phosphorothioation.International journal of molecular sciences · 2025Article
- Reappraisal of the DNA phosphorothioate modification machinery: uncovering neglected functional modalities and identification of new counter-invader defense systems.Nucleic acids research · 2024Article
- Article
- Nanopore Sequencing for Detection and Characterization of Phosphorothioate Modifications in Native DNA Sequences.Frontiers in microbiology · 2022Article
- The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes.Nature communications · 2021Article
- Structural Analysis of an l-Cysteine Desulfurase from an Ssp DNA Phosphorothioation System.mBio · 2020Article
- Phosphorothioated DNA Is Shielded from Oxidative Damage.Applied and environmental microbiology · 2019Article
- A new type of DNA phosphorothioation-based antiviral system in archaea.Nature communications · 2019Article
- DNA phosphorothioate modification-a new multi-functional epigenetic system in bacteria.FEMS microbiology reviews · 2019Review
- Structural basis for the recognition of sulfur in phosphorothioated DNA.Nature communications · 2018Article
- Occurrence, evolution, and functions of DNA phosphorothioate epigenetics in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Evolutionary Genomics of Defense Systems in Archaea and Bacteria.Annual review of microbiology · 2017Review
- Mechanistic Investigation on ROS Resistance of Phosphorothioated DNA.Scientific reports · 2017Article
- Structural investigation into physiological DNA phosphorothioate modification.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA phosphorothioation (PT) is the first discovered physiological DNA backbone modification, in which a non-bridging oxygen atom of the phosphodiester bond is replaced with a sulfur atom in Rp (rectus for plane) configuration. PT modification is governed by a highly conserved gene cluster dndA/iscS-dndBCDE that is widespread across bacterial and archaeal species. However, little is known about how these proteins coordinately react with each other to perform oxygen-sulfur swap. We here demonstrated that IscS, DndC, DndD and DndE form a protein complex of which the molecular ratio for four proteins in the complex is approximate 1:1:1:1. DndB here displayed little or weak affinity to the complex and the constructs harboring dndACDE can confer the host in vivo PT modification. Using co-purification and pull down strategy, we demonstrated that the four proteins assemble into a pipeline in collinear to its gene organization, namely, IscS binding to DndC, DndC binding to DndD, and DndD binding to DndE. Moreover, weak interactions between DndE and IscS, DndE and DndC were also identified.
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