ArticleJournal of biomedical optics2015
Differences in forward angular light scattering distributions between M1 and M2 macrophages.
Article in Journal of biomedical optics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Development of molecular sensors based on fluorescent proteins for polarized macrophages identification.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Single cell classification of macrophage subtypes by label-free cell signatures and machine learning.Royal Society open science · 2022Article
- Single cell sequencing unraveling genetic basis of severe COVID19 in obesity.Obesity medicine · 2020Review
- Classification and Recognition of Ovarian Cells Based on Two-Dimensional Light Scattering Technology.Journal of medical systems · 2019Article
- Classification of M1/M2-polarized human macrophages by label-free hyperspectral reflectance confocal microscopy and multivariate analysis.Scientific reports · 2017Article
- Label-free identification of macrophage phenotype by fluorescence lifetime imaging microscopy.Journal of biomedical optics · 2016Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The ability to distinguish macrophage subtypes noninvasively could have diagnostic potential in cancer, atherosclerosis, and diabetes, where polarized M1 and M2 macrophages play critical and often opposing roles. Current methods to distinguish macrophage subtypes rely on tissue biopsy. Optical imaging techniques based on light scattering are of interest as they can be translated into biopsy-free strategies. Because mitochondria are relatively strong subcellular light scattering centers, and M2 macrophages are known to have enhanced mitochondrial biogenesis compared to M1, we hypothesized that M1 and M2 macrophages may have different angular light scattering profiles. To test this, we developed an in vitro angle-resolved forward light scattering measurement system. We found that M1 and M2 macrophage monolayers scatter relatively unequal amounts of light in the forward direction between 1.6 deg and 3.2 deg with M2 forward scattering significantly more light than M1 at increasing angles. The ratio of forward scattering can be used to identify the polarization state of macrophage populations in culture.
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Registered trials
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