Evidence map›Paper›PMID 26537830›Full record

ArticleHypertension research : official journal of the Japanese Society of Hypertension2016

Endothelial function does not improve with high-intensity continuous exercise training in SHR: implications of eNOS uncoupling.

Sylvain Battault, François Singh, Sandrine Gayrard, Joffrey Zoll, Cyril Reboul, Grégory Meyer

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In one paragraph

Article in Hypertension research : official journal of the Japanese Society of Hypertension, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 1 pooled it
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.

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  20. Moderate-intensity exercise allows enhanced protection against oxidative stress-induced cardiac dysfunction in spontaneously hypertensive rats.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2019
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Sylvain BattaultLaboratoire de Pharm-Ecologie Cardiovasculaire (EA4278), Faculty of Sciences, Avignon University, Avignon, France.
François SinghE.A. 3072, Fédération de Médecine Translationnelle, Faculty of Medicine, University of Strasbourg, Strasbourg, France.
Sandrine GayrardLaboratoire de Pharm-Ecologie Cardiovasculaire (EA4278), Faculty of Sciences, Avignon University, Avignon, France.
Joffrey ZollE.A. 3072, Fédération de Médecine Translationnelle, Faculty of Medicine, University of Strasbourg, Strasbourg, France.
Cyril ReboulLaboratoire de Pharm-Ecologie Cardiovasculaire (EA4278), Faculty of Sciences, Avignon University, Avignon, France.
Grégory MeyerLaboratoire de Pharm-Ecologie Cardiovasculaire (EA4278), Faculty of Sciences, Avignon University, Avignon, France.
Université d'Avignon et des Pays de Vaucluse · FRUniversité de Strasbourg · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exercise training is a well-recognized way to improve vascular endothelial function by increasing nitric oxide (NO) bioavailability. However, in hypertensive subjects, unlike low- and moderate-intensity exercise training, the beneficial effects of continuous high-intensity exercise on endothelial function are not clear, and the underlying mechanisms remain unknown. The aim of this study was to investigate the impact of high-intensity exercise on vascular function, especially on the NO pathway, in spontaneous hypertensive rats (SHR). These effects were studied on WKY, sedentary SHR and SHR that exercised at moderate (SHR-MOD) and high intensity (SHR-HI) on a treadmill (1 h per day; 5 days per week for 6 weeks at 55% and 80% of their maximal aerobic velocity, respectively). Endothelial function and specific NO contributions to acetylcholine-mediated relaxation were evaluated by measuring the aortic ring isometric forces. Endothelial nitric oxide synthase (eNOS) expression and phosphorylation (ser1177) were evaluated by western blotting. The total aortic and eNOS-dependent reactive oxygen species (ROS) production was assessed using electron paramagnetic resonance in aortic tissue. Although the aortas of SHR-HI had increased eNOS levels without alteration of eNOS phosphorylation, high-intensity exercise had no beneficial effect on endothelium-dependent vasorelaxation, unlike moderate exercise. This result was associated with increased eNOS-dependent ROS production in the aortas of SHR-HI. Notably, the use of the recoupling agent BH4 or a thiol-reducing agent blunted eNOS-dependent ROS production in the aortas of SHR-HI. In conclusion, the lack of a positive effect of high-intensity exercise on endothelial function in SHR was mainly explained by redox-dependent eNOS uncoupling, resulting in a switch from NO to O2(-) generation.

Indexed as

Physical Conditioning, AnimalAcetylcholineAnaerobic ThresholdAnimalsAorta, ThoracicEndothelium, VascularHypertensionIsometric ContractionMaleNitric OxideNitric Oxide Synthase Type IIIRatsRats, Inbred SHRRats, Inbred WKYReactive Oxygen SpeciesVasodilationAcetylcholineNitric OxideNitric Oxide Synthase Type IIINos3 protein, ratReactive Oxygen SpeciesVasodilator Agents

Identifiers

PMID26537830
OpenAlexW2217139260

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.