ArticleJournal of applied oral science : revista FOB2015
Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells.
Article in Journal of applied oral science : revista FOB, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- The evaluation of MTA and Biodentine as a pulpotomy materials for carious exposures in primary teeth.Clinical oral investigations · 2019Trial
- Cryotherapy-Driven Modulation of Postoperative Pain in Single-Visit Endodontic Treatment Across Different Obturation Materials: A Retrospective Study.Journal of clinical medicine · 2026Article
- Cytotoxicity of Root Canal Sealers and Potential Clinical Implications: A Comprehensive Systematic Review of In Vitro Studies.Journal of clinical medicine · 2026Review
- In Vitro Microscopical and Microbiological Assessment of the Sealing Ability of Calcium Silicate-Based Root Canal Sealers.Journal of functional biomaterials · 2024Article
- Ex Vivo Osteogenesis Induced by Calcium Silicate-Based Cement Extracts.Journal of functional biomaterials · 2023Article
- Cytotoxicity comparison of Bio C Sealer against multiple root canal sealers.Journal of clinical and experimental dentistry · 2023Article
- Bioactivity Potential of Bioceramic-Based Root Canal Sealers: A Scoping Review.Life (Basel, Switzerland) · 2022Article
- Physicochemical Properties and Inductive Effect of Calcium Strontium Silicate on the Differentiation of Human Dental Pulp Stem Cells for Vital Pulp Therapies: An In Vitro Study.Materials (Basel, Switzerland) · 2022Article
- Review
- The role of NMT induction on odontogenic proliferation and differentiation of dental pulp stem cells.Heliyon · 2021Article
- GuttaFlowMolecules (Basel, Switzerland) · 2020Article
- Article
- Cytotoxicity and Effects of a New Cacium Hydroxide Nanoparticle Material on Production of Reactive Oxygen Species by LPS-Stimulated Dental Pulp Cells.Iranian endodontic journal · 2020Article
- Bioactivity of Bioceramic Materials Used in the Dentin-Pulp Complex Therapy: A Systematic Review.Materials (Basel, Switzerland) · 2019Review
- Evaluation of the biocompatibility of root canal sealers on human periodontal ligament cells ex vivo.Odontology · 2019Article
- Effect of root canal sealers on human periodontal ligament fibroblast viability: ex vivo study.Odontology · 2018Article
- Preferences of dentists and endodontists, in Saudi Arabia, on management of necrotic pulp with acute apical abscess.BMC oral health · 2018Article
- Article
- Composition and physicochemical properties of calcium silicate based sealers: A review article.Journal of clinical and experimental dentistry · 2017Review
- Zinc-Containing Hydroxyapatite Enhances Cold-Light-Activated Tooth Bleaching Treatment In Vitro.BioMed research international · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledMineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus.
objectiveThe aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs). MATERIAL AND
methodsThe cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP); MTA Fillapex (MTAF) and FillCanal (FC). Biocompatibility was evaluated with MTT and Neutral Red (NR) assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4). Unexposed cells were the positive control (CT). Bioactivity was assessed by alkaline phosphatase (ALP) enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution). All data were analyzed by ANOVA and Tukey post-test (p≤0.05%).
resultsMTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%). Cells exposed to MTAF and FC (1:2 and 1:4 dilutions) showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure.
conclusionsThe hDPCs were suitable for the evaluation of new endodontic materialsin vitro. MTAP may be considered a promising material for endodontic treatments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.