ArticleBMC genomics2015
Comparative genome analysis identifies two large deletions in the genome of highly-passaged attenuated Streptococcus agalactiae strain YM001 compared to the parental pathogenic strain HN016.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 38 citations in OpenAlex.
- Type VII secretion system promotes Streptococcus agalactiae virulence through magnesium acquisition and capsule maintenance.Veterinary research · 2026Article
- CRISPR-Cas9-mediated construction of a Streptococcus agalactiae vaccine for tilapia and evaluation of its protective efficacy.BMC veterinary research · 2026Article
- CorAVeterinary research · 2026Article
- Evidence of a novel sublineage ofMicrobial genomics · 2025Article
- Identification of ClpB, a molecular chaperone involved in the stress tolerance and virulence of Streptococcus agalactiae.Veterinary research · 2024Article
- Article
- Research Advances on Tilapia Streptococcosis.Pathogens (Basel, Switzerland) · 2021Review
- Arginine Deiminase and Biotin Metabolism Signaling Pathways Play an Important Role in Human-Derived Serotype V, ST1 Streptococcus agalactiae Virulent Strain upon Infected Tilapia.Animals : an open access journal from MDPI · 2020Article
- Transcriptome Analysis of Juvenile Tilapia (Frontiers in physiology · 2020Article
- Live vaccines against bacterial fish diseases: A review.Veterinary world · 2019Review
- The Interaction between Phagocytes andAnimals : an open access journal from MDPI · 2019Article
- Article
- Effects of AttenuatedFrontiers in microbiology · 2018Article
- Genome comparison of different Zymomonas mobilis strains provides insights on conservation of the evolution.PloS one · 2018Article
- Group B Streptococcus Infections Caused by Improper Sourcing and Handling of Fish for Raw Consumption, Singapore, 2015-2016.Emerging infectious diseases · 2017Article
- Aquaculture genomics, genetics and breeding in the United States: current status, challenges, and priorities for future research.BMC genomics · 2017Review
- Review
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStreptococcus agalactiae (S. agalactiae), also known as group B Streptococcus (GBS), is an important pathogen for neonatal pneumonia, meningitis, bovine mastitis, and fish meningoencephalitis. The global outbreaks of Streptococcus disease in tilapia cause huge economic losses and threaten human food hygiene safety as well. To investigate the mechanism of S. agalactiae pathogenesis in tilapia and develop attenuated S. agalactiae vaccine, this study sequenced and comparatively analyzed the whole genomes of virulent wild-type S. agalactiae strain HN016 and its highly-passaged attenuated strain YM001 derived from tilapia.
methodsWe performed Illumina sequencing of DNA prepared from strain HN016 and YM001. Sequencedreads were assembled and nucleotide comparisons, single nucleotide polymorphism (SNP) , indels were analyzed between the draft genomes of HN016 and YM001. Clustered regularly interspaced short palindromic repeats (CRISPRs) and prophage were detected and analyzed in different S. agalactiae strains.
resultsThe genome of S. agalactiae YM001 was 2,047,957 bp with a GC content of 35.61 %; it contained 2044 genes and 88 RNAs. Meanwhile, the genome of S. agalactiae HN016 was 2,064,722 bp with a GC content of 35.66 %; it had 2063 genes and 101 RNAs. Comparative genome analysis indicated that compared with HN016, YM001 genome had two significant large deletions, at the sizes of 5832 and 11,116 bp respectively, resulting in the deletion of three rRNA and ten tRNA genes, as well as the deletion and functional damage of ten genes related to metabolism, transport, growth, anti-stress, etc. Besides these two large deletions, other ten deletions and 28 single nucleotide variations (SNVs) were also identified, mainly affecting the metabolism- and growth-related genes.
conclusionsThe genome of attenuated S. agalactiae YM001 showed significant variations, resulting in the deletion of 10 functional genes, compared to the parental pathogenic strain HN016. The deleted and mutated functional genes all encode metabolism- and growth-related proteins, not the known virulence proteins, indicating that the metabolism- and growth-related genes are important for the pathogenesis of S. agalactiae.
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