ArticleGenetics and molecular biology2015
Acetosyringone, pH and temperature effects on transient genetic transformation of immature embryos of Brazilian wheat genotypes by Agrobacterium tumefaciens.
Article in Genetics and molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Establishment ofInternational journal of molecular sciences · 2024Article
- Agrobacterium tumefaciens-Mediated Plant Transformation: A Review.Molecular biotechnology · 2024Review
- Reasons and riddance of Agrobacterium tumefaciens overgrowth in plant transformation.Transgenic research · 2023Review
- A New Approach for ControllingPlants (Basel, Switzerland) · 2022Article
- Optimizing Transformation Frequency ofJournal of fungi (Basel, Switzerland) · 2021Review
- Establishment of3 Biotech · 2021Article
- Review
- Phenotyping of VIGS-mediated gene silencing in rice using a vector derived from a DNA virus.Plant cell reports · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low transformation efficiency is one of the main limiting factors in the establishment of genetic transformation of wheat via Agrobacterium tumefaciens. To determine more favorable conditions for T-DNA delivery and explant regeneration after infection, this study investigated combinations of acetosyringone concentration and pH variation in the inoculation and co-cultivation media and co-culture temperatures using immature embryos from two Brazilian genotypes (BR 18 Terena and PF 020037). Based on transient expression of uidA, the most favorable conditions for T-DNA delivery were culture media with pH 5.0 and 5.4 combined with co-culture temperatures of 22 °C and 25 °C, and a 400 μM acetosyringone supplement. These conditions resulted in blue foci in 81% of the embryos. Media with more acidic pH also presented reduced A. tumefaciens overgrowth during co-culture, and improved regeneration frequency of the inoculated explants. BR 18 Terena was more susceptible to infection by A. tumefaciens than PF 020037. We found that it is possible to improve T-DNA delivery and explant regeneration by adjusting factors involved in the early stages of A. tumefaciens infection. This can contribute to establishing a stable transformation procedure in the future.
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Registered trials
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