ArticleScientific reports2015
Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- Clinical management of snakebite envenoming: Future perspectives.Toxicon: X · 2021Article
- Snakebite Envenoming Diagnosis and Diagnostics.Frontiers in immunology · 2021Review
- Multiplex Ligation-Dependent Probe Amplification for Simultaneous Identification ofFrontiers in pharmacology · 2020Article
- Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR.Scientific reports · 2017Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. The identification of medicinal snakes in patented Chinese drugs was successfully performed using this detection system. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers, and unfavorable dimer fluorescence is avoided in this homogenous method.
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