ArticleScientific reports2015
A transgenic embryonic sexing system for the Australian sheep blow fly Lucilia cuprina.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 87 citations in OpenAlex.
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- Synthetic biology approaches to generate temperature-sensitive alleles for the Sterile Insect Technique.Insect science · 2026Review
- Selection, counterselection, and conditional gene expression for genetic biocontrol of insects.Insect science · 2026Review
- The transformer gene controls sexual development in Drosophila suzukii.Insect science · 2026Article
- Improving the sex-specificity of a conditional female lethal system for genetic biocontrol of the New World screwworm, Cochliomyia hominivorax.Scientific reports · 2025Article
- Direct and transgenerational effects of tetracyclines on the microbiome, transcriptome, and male mating behavior of the sheep blowfly Lucilia cuprina.G3 (Bethesda, Md.) · 2025Article
- Genetic and behavioral analyses suggest that larval and adult stages of Lucilia cuprina employ different sensory systems to detect rotten beef.Parasites & vectors · 2025Article
- Antennal transcriptome analysis reveals sensory receptors potentially associated with host detection in the livestock pest Lucilia cuprina.Parasites & vectors · 2024Article
- Effects of antibiotics on theFrontiers in bioengineering and biotechnology · 2023Article
- Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression.eLife · 2022Article
- Control of sheep flystrike: what's been tried in the past and where to from here.Australian veterinary journal · 2022Review
- Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression.PLoS genetics · 2021Article
- The βScientific reports · 2021Article
- A transgenic female killing system for the genetic control of Drosophila suzukii.Scientific reports · 2021Article
- Mathematical modeling of genetic pest management through female-specific lethality: Is one locus better than two?Evolutionary applications · 2021Article
- Review
- Identification and characterization of four Drosophila suzukii cellularization genes and their promoters.BMC genetics · 2020Article
- Improvement on the genetic engineering of an invasive agricultural pest insect, the cherry vinegar fly, Drosophila suzukii.BMC genetics · 2020Article
- Building a transgenic sexing strain for genetic control of the Australian sheep blow fly Lucilia cuprina using two lethal effectors.BMC genetics · 2020Article
- An early female lethal system of the New World screwworm, Cochliomyia hominivorax, for biotechnology-enhanced SIT.BMC genetics · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic approaches, including the sterile insect technique (SIT), have previously been considered for control of the Australian sheep blow fly Lucilia cuprina, a major pest of sheep. In an SIT program, females consume 50% of the diet but are ineffective as control agents and compete with females in the field for mating with sterile males, thereby decreasing the efficiency of the program. Consequently, transgenic sexing strains of L. cuprina were developed that produce 100% males when raised on diet that lacks tetracycline. However, as females die mostly at the pupal stage, rearing costs would not be significantly reduced. Here we report the development of transgenic embryonic sexing strains of L. cuprina. In these strains, the Lsbnk cellularization gene promoter drives high levels of expression of the tetracycline transactivator (tTA) in the early embryo. In the absence of tetracycline, tTA activates expression of the Lshid proapoptotic gene, leading to death of the embryo. Sex-specific RNA splicing of Lshid transcripts ensures that only female embryos die. Embryonic sexing strains were also made by combining the Lsbnk-tTA and tetO-Lshid components into a single gene construct, which will facilitate transfer of the technology to other major calliphorid livestock pests.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.