ArticleBiophysical journal2015
Predictive Spatiotemporal Manipulation of Signaling Perturbations Using Optogenetics.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed.
- Modeling mechanochemical coupling in optogenetically activated cell layers.Biophysical journal · 2026Article
- Force propagation between epithelial cells depends on active coupling and mechano-structural polarization.eLife · 2023Article
- Actuation of single downstream nodes in growth factor network steers immune cell migration.Developmental cell · 2023Article
- Cell size and actin architecture determine force generation in optogenetically activated cells.Biophysical journal · 2023Article
- Light-driven biological actuators to probe the rheology of 3D microtissues.Nature communications · 2023Article
- Optogenetic modulation of guanine nucleotide exchange factors of Ras superfamily proteins directly controls cell shape and movement.Frontiers in cell and developmental biology · 2023Article
- Optogenetic manipulation of cell migration with high spatiotemporal resolution using lattice lightsheet microscopy.Communications biology · 2022Article
- Computational framework for single-cell spatiotemporal dynamics of optogenetic membrane recruitment.Cell reports methods · 2022Article
- Spindle reorientation in response to mechanical stress is an emergent property of the spindle positioning mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking.eLife · 2022Article
- A small and highly sensitive red/far-red optogenetic switch for applications in mammals.Nature biotechnology · 2022Article
- Designing Single-Component Optogenetic Membrane Recruitment Systems: The Rho-Family GTPase Signaling Toolbox.ACS synthetic biology · 2022Article
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
- The Promise of Optogenetics for Bioproduction: Dynamic Control Strategies and Scale-Up Instruments.Bioengineering (Basel, Switzerland) · 2020Review
- Parallelized Manipulation of Adherent Living Cells by Magnetic Nanoparticles-Mediated Forces.International journal of molecular sciences · 2020Article
- The Actin Cytoskeleton as an Active Adaptive Material.Annual review of condensed matter physics · 2020Article
- Small GTPases orchestrate cell-cell communication during collective cell movement.Small GTPases · 2020Review
- Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2020Article
- Stick-slip dynamics of cell adhesion triggers spontaneous symmetry breaking and directional migration of mesenchymal cells on one-dimensional lines.Science advances · 2020Article
- Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently developed optogenetic methods promise to revolutionize cell biology by allowing signaling perturbations to be controlled in space and time with light. However, a quantitative analysis of the relationship between a custom-defined illumination pattern and the resulting signaling perturbation is lacking. Here, we characterize the biophysical processes governing the localized recruitment of the Cryptochrome CRY2 to its membrane-anchored CIBN partner. We develop a quantitative framework and present simple procedures that enable predictive manipulation of protein distributions on the plasma membrane with a spatial resolution of 5 μm. We show that protein gradients of desired levels can be established in a few tens of seconds and then steadily maintained. These protein gradients can be entirely relocalized in a few minutes. We apply our approach to the control of the Cdc42 Rho GTPase activity. By inducing strong localized signaling perturbation, we are able to monitor the initiation of cell polarity and migration with a remarkable reproducibility despite cell-to-cell variability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.