ArticleThe Journal of biological chemistry2015
Elucidating the Specificity Determinants of the AtxE2 Lasso Peptide Isopeptidase.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Tangled Tail of Mechanically Interlocked Peptides.Journal of the American Chemical Society · 2026Review
- Biosynthesis, Structure, and Antibiotic Properties of Gelatinamin A, a Triculamin-Like Lasso Peptide.Chembiochem : a European journal of chemical biology · 2026Article
- Advances in lasso peptide discovery, biosynthesis, and function.Trends in genetics : TIG · 2024Review
- Unusual Post-Translational Modifications in the Biosynthesis of Lasso Peptides.International journal of molecular sciences · 2022Review
- Comprehensive Mutational Analysis of the Lasso Peptide Klebsidin.ACS chemical biology · 2022Article
- Rational generation of lasso peptides based on biosynthetic gene mutations and site-selective chemical modifications.Chemical science · 2021Article
- How to harness biosynthetic gene clusters of lasso peptides.Journal of industrial microbiology & biotechnology · 2020Review
- Discovery of Ubonodin, an Antimicrobial Lasso Peptide Active against Members of the Burkholderia cepacia Complex.Chembiochem : a European journal of chemical biology · 2020Article
- Lasso Peptides: Heterologous Production and Potential Medical Application.Frontiers in bioengineering and biotechnology · 2020Review
- Pandonodin: A Proteobacterial Lasso Peptide with an Exceptionally Long C-Terminal Tail.ACS chemical biology · 2019Article
- Genome mining for lasso peptides: past, present, and future.Journal of industrial microbiology & biotechnology · 2019Review
- Heterologous and in Vitro Reconstitution of Fuscanodin, a Lasso Peptide from Thermobifida fusca.Journal of the American Chemical Society · 2019Article
- Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin.Chemical communications (Cambridge, England) · 2018Article
- Lasso Peptide Benenodin-1 Is a Thermally Actuated [1]Rotaxane Switch.Journal of the American Chemical Society · 2017Article
- Structure of the Lasso Peptide Isopeptidase Identifies a Topology for Processing Threaded Substrates.Journal of the American Chemical Society · 2016Article
- Thermal Unthreading of the Lasso Peptides Astexin-2 and Astexin-3.ACS chemical biology · 2016Article
- The B1 Protein Guides the Biosynthesis of a Lasso Peptide.Scientific reports · 2016Article
- In Vitro Biosynthesis and Substrate Tolerance of the Plantazolicin Family of Natural Products.ACS chemical biology · 2016Article
- Insights into the Unique Phosphorylation of the Lasso Peptide Paeninodin.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
Lasso peptide isopeptidase is an enzyme that specifically hydrolyzes the isopeptide bond of lasso peptides, rendering these peptides linear. To carry out a detailed structure-activity analysis of the lasso peptide isopeptidase AtxE2 from Asticcacaulis excentricus, we solved NMR structures of its substrates astexin-2 and astexin-3. Using in vitro enzyme assays, we show that the C-terminal tail portion of these peptides is dispensable with regards to isopeptidase activity. A collection of astexin-2 and astexin-3 variants with alanine substitutions at each position within the ring and the loop was constructed, and we showed that all of these peptides except for one were cleaved by the isopeptidase. Thus, much like the lasso peptide biosynthetic enzymes, lasso peptide isopeptidase has broad substrate specificity. Quantitative analysis of the cleavage reactions indicated that alanine substitutions in loop positions of these peptides led to reduced cleavage, suggesting that the loop is serving as a recognition element for the isopeptidase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.