ArticleGenetics2016
The Splice Isoforms of the Drosophila Ecdysis Triggering Hormone Receptor Have Developmentally Distinct Roles.
Article in Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- Phase-Resolved Pupal Ecdysis Behavior and Requirement of Ecdysis-Triggering Hormone in Henosepilachna vigintioctomaculata.Archives of insect biochemistry and physiology · 2026Article
- Characterization of eclosion hormone receptor function reveals differential hormonal control of ecdysis during Drosophila development.PLoS genetics · 2025Article
- Characterization Eclosion Hormone Receptor function reveals differential hormonal control of ecdysis duringbioRxiv : the preprint server for biology · 2025Article
- Neuromodulation and the toolkit for behavioural evolution: can ecdysis shed light on an old problem?The FEBS journal · 2024Review
- Review
- Extracting temporal relationships between weakly coupled peptidergic and motoneuronal signaling: Application to Drosophila ecdysis behavior.PLoS computational biology · 2021Article
- Pupal behavior emerges from unstructured muscle activity in response to neuromodulation ineLife · 2021Article
- Identification and function of ETH receptor networks in the silkworm Bombyx mori.Scientific reports · 2021Article
- Genome-wide identification of neuropeptides and their receptor genes in Bemisia tabaci and their transcript accumulation change in response to temperature stresses.Insect science · 2021Article
- Article
- Functional Characterization of Ecdysis Triggering Hormone Receptors (AgETHR-A and AgETHR-B) in the African Malaria Mosquito,Frontiers in physiology · 2021Article
- Regulation ofFrontiers in neuroscience · 2021Article
- Article
- TheFrontiers in neural circuits · 2020Review
- Two Splice Isoforms ofFrontiers in physiology · 2020Article
- Article
- Ultraspiracle-independent anti-apoptotic function of ecdysone receptors is required for the survival of larval peptidergic neurons via suppression of grim expression in Drosophila melanogaster.Apoptosis : an international journal on programmed cell death · 2019Article
- Transcriptomic analysis of crustacean neuropeptide signaling during the moult cycle in the green shore crab, Carcinus maenas.BMC genomics · 2018Article
- A gene-specificeLife · 2018Article
- Endocrine regulation of airway clearance inProceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
To grow, insects must periodically shed their exoskeletons. This process, called ecdysis, is initiated by the endocrine release of Ecdysis Trigger Hormone (ETH) and has been extensively studied as a model for understanding the hormonal control of behavior. Understanding how ETH regulates ecdysis behavior, however, has been impeded by limited knowledge of the hormone's neuronal targets. An alternatively spliced gene encoding a G-protein-coupled receptor (ETHR) that is activated by ETH has been identified, and several lines of evidence support a role in ecdysis for its A-isoform. The function of a second ETHR isoform (ETHRB) remains unknown. Here we use the recently introduced "Trojan exon" technique to simultaneously mutate the ETHR gene and gain genetic access to the neurons that express its two isoforms. We show that ETHRA and ETHRB are expressed in largely distinct subsets of neurons and that ETHRA- but not ETHRB-expressing neurons are required for ecdysis at all developmental stages. However, both genetic and neuronal manipulations indicate an essential role for ETHRB at pupal and adult, but not larval, ecdysis. We also identify several functionally important subsets of ETHR-expressing neurons including one that coexpresses the peptide Leucokinin and regulates fluid balance to facilitate ecdysis at the pupal stage. The general strategy presented here of using a receptor gene as an entry point for genetic and neuronal manipulations should be useful in establishing patterns of functional connectivity in other hormonally regulated networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.