ArticleNucleic acids research2016
Domain swapping between homologous bacterial small RNAs dissects processing and Hfq binding determinants and uncovers an aptamer for conditional RNase E cleavage.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Evaluation of 5'-End Phosphorylation for Small RNA Stability and Target Regulation In Vivo.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Role of the 5' end phosphorylation state for small RNA stability and target RNA regulation in bacteria.Nucleic acids research · 2023Article
- Structure of a bacterial ribonucleoprotein complex central to the control of cell envelope biogenesis.The EMBO journal · 2023Article
- The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches.Frontiers in molecular biosciences · 2021Review
- Adaptor protein RapZ activates endoribonuclease RNase E by protein-protein interaction to cleave a small regulatory RNA.RNA (New York, N.Y.) · 2020Article
- Small RNA-binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli.The EMBO journal · 2020Article
- Trans-Acting Small RNAs and Their Effects on Gene Expression inEcoSal Plus · 2020Review
- The bacterial endoribonuclease RNase E can cleave RNA in the absence of the RNA chaperone Hfq.The Journal of biological chemistry · 2019Article
- Posttranscription Initiation Control of Gene Expression Mediated by Bacterial RNA-Binding Proteins.Annual review of microbiology · 2019Review
- Feedback regulation of small RNA processing by the cleavage product.RNA biology · 2019Article
- Stem-loops direct precise processing of 3' UTR-derived small RNA MicL.Nucleic acids research · 2019Article
- Interaction of lipoprotein QseG with sensor kinase QseE in the periplasm controls the phosphorylation state of the two-component system QseE/QseF in Escherichia coli.PLoS genetics · 2018Article
- Carbohydrate Utilization in Bacteria: Making the Most Out of Sugars with the Help of Small Regulatory RNAs.Microbiology spectrum · 2018Review
- Structural insights into RapZ-mediated regulation of bacterial amino-sugar metabolism.Nucleic acids research · 2017Article
- Effect of Crc and Hfq proteins on the transcription, processing, and stability of the Pseudomonas putida CrcZ sRNA.RNA (New York, N.Y.) · 2016Article
- Visualized and precise design of artificial small RNAs for regulating T7 RNA polymerase and enhancing recombinant protein folding inSynthetic and systems biotechnology · 2016Article
- Antagonistic control of the turnover pathway for the global regulatory sRNA CsrB by the CsrA and CsrD proteins.Nucleic acids research · 2016Article
- Two Small RNAs Conserved in Enterobacteriaceae Provide Intrinsic Resistance to Antibiotics Targeting the Cell Wall Biosynthesis Enzyme Glucosamine-6-Phosphate Synthase.Frontiers in microbiology · 2016Article
Corrections and comments
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3 authors at 2 institutions in 1 country.
Funding
Abstract
In E. coli, small RNA GlmZ activates the glmS mRNA by base-pairing in an Hfq dependent manner. When not required, GlmZ is bound by adaptor protein RapZ and recruited to RNase E, which cleaves GlmZ in its base-pairing sequence. Small RNA GlmY counteracts cleavage of GlmZ by sequestration of RapZ. Although both sRNAs are highly homologous, only GlmZ specifically binds Hfq and undergoes cleavage by RNase E. We used domain swapping to identify the responsible modules. Two elements, the 3' terminal oligo(U) stretch and the base-pairing region enable GlmZ to interact with Hfq. Accordingly, Hfq inhibits cleavage of GlmZ, directing it to base-pairing. Intriguingly, the central stem loop of GlmZ is decisive for cleavage, whereas the sequence comprising the actual cleavage site is dispensable. Assisted by RapZ, RNase E cleaves any RNA fused to the 3' end of this module. These results suggest a novel mode for RNase E recognition, in which one of the required handholds in the substrate is replaced by an RNA binding protein. This device can generate RNAs of interest in their 5' monophosphorylated form on demand. As these species are rapidly degraded, this tool allows to regulate gene expression post-transcriptionally by modulation of RapZ levels.
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