ArticleNucleic acids research2015
Collaborator of alternative reading frame protein (CARF) regulates early processing of pre-ribosomal RNA by retaining XRN2 (5'-3' exoribonuclease) in the nucleoplasm.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Proteomic Approach Using DIA-MS Identifies Morphogenesis-Associated Proteins during Cardiac Differentiation of Human iPS Cells.ACS omega · 2025Article
- Fragile X Messenger Ribonucleoprotein Protein and Its Multifunctionality: From Cytosol to Nucleolus and Back.Biomolecules · 2024Review
- Mitochondrion-targeted RNA therapies as a potential treatment strategy for mitochondrial diseases.Molecular therapy. Nucleic acids · 2022Review
- CDKN2AIP is critical for spermiogenesis and germ cell development.Cell & bioscience · 2022Article
- Nuclear RNA Regulation by XRN2 and XTBD Family Proteins.Cell structure and function · 2021Review
- Non-coding RNA Regulated Cross-Talk Between Mitochondria and Other Cellular Compartments.Frontiers in cell and developmental biology · 2021Review
- Non-Coding RNA-Driven Regulation of rRNA Biogenesis.International journal of molecular sciences · 2020Review
- CARF promotes spermatogonial self-renewal and proliferation through Wnt signaling pathway.Cell discovery · 2020Article
- XRN2 interactome reveals its synthetic lethal relationship with PARP1 inhibition.Scientific reports · 2020Article
- Stress-induced changes in CARF expression determine cell fate to death, survival, or malignant transformation.Cell stress & chaperones · 2020Article
- MicroRNAs and long non-coding RNAs as novel regulators of ribosome biogenesis.Biochemical Society transactions · 2020Review
- Full-length NF-κB repressing factor contains an XRN2 binding domain.The Biochemical journal · 2020Article
- IT'S 2 for the price of 1: Multifaceted ITS2 processing machines in RNA and DNA maintenance.DNA repair · 2019Review
- SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation.Nature structural & molecular biology · 2018Article
- Noncoding RNA Surveillance: The Ends Justify the Means.Chemical reviews · 2018Review
- The G-patch protein NF-κB-repressing factor mediates the recruitment of the exonuclease XRN2 and activation of the RNA helicase DHX15 in human ribosome biogenesis.Nucleic acids research · 2017Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Collaborator of alternative reading frame protein (CARF) associates directly with ARF, p53, and/or human double minute 2 protein (HDM2), a ubiquitin-protein ligase, without cofactors and regulates cell proliferation by forming a negative feedback loop. Although ARF, p53, and HDM2 also participate in the regulation of ribosome biogenesis, the involvement of CARF in this process remains unexplored. In this study, we demonstrate that CARF associates with 5'-3' exoribonuclease 2 (XRN2), which plays a major role in both the maturation of rRNA and the degradation of a variety of discarded pre-rRNA species. We show that overexpression of CARF increases the localization of XRN2 in the nucleoplasm and a concomitant suppression of pre-rRNA processing that leads to accumulation of the 5' extended from of 45S/47S pre-rRNA and 5'-01, A0-1 and E-2 fragments of pre-rRNA transcript in the nucleolus. This was also observed upon XRN2 knockdown. Knockdown of CARF increased the amount of XRN2 in the nucleolar fraction as determined by cell fractionation and by immnocytochemical analysis. These observations suggest that CARF regulates early steps of pre-rRNA processing during ribosome biogenesis by controlling spatial distribution of XRN2 between the nucleoplasm and nucleolus.
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