Evidence map›Paper›PMID 26531772›Full record

ArticleBiointerphases2015

Three-dimensional localization of polymer nanoparticles in cells using ToF-SIMS.

Daniel J Graham, John T Wilson, James J Lai, Patrick S Stayton, David G Castner

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Biointerphases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Daniel J GrahamNational ESCA and Surface Analysis Center for Biomedical Problems, Seattle, Washington, 98195 and Department of Bioengineering, University of Washington, Seattle, Washington 98195.
John T WilsonDepartment of Bioengineering, University of Washington, Seattle, Washington 98195 and Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, Tennessee 37212.
James J LaiDepartment of Bioengineering, University of Washington, Seattle, Washington 98195.
Patrick S StaytonDepartment of Bioengineering, University of Washington, Seattle, Washington 98195.
David G CastnerNational ESCA and Surface Analysis Center for Biomedical Problems, Seattle, Washington, 98195; Department of Bioengineering, University of Washington, Seattle, Washington 98195; and Department of Chemical Engineering, University of Washington, Seattle, Washington 98185.

Funding

Surface Analysis Facility for Biomedical ProblemsP41EB002027 · NIBIB · UNIVERSITY OF WASHINGTON · PI CASTNER, DAVID G, GAMBLE, LARA · 2003 to 2019
$17.6M
NIBIB NIH HHS EB-002027NIBIB NIH HHS P41 EB002027
6 · The paper itself

Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) three-dimensional (3D) depth profiling and a novel background subtraction method were used to localize polymeric nanoparticles within cells. Results showed that ToF-SIMS 3D depth profiling is capable of localizing polymer nanoparticles within HeLa cells. ToF-SIMS results compared well with optical images of cells incubated with fluorescently labeled polymer nanoparticles, with both imaging techniques demonstrating clustering of nanoparticles in punctate regions consistent with endosomal localization as anticipated based on the nanoparticle design.

Indexed as

Epithelial CellsHeLa CellsHumansImaging, Three-DimensionalNanoparticlesPolymersSpectrometry, Mass, Secondary IonPolymers

Identifiers

PMID26531772
PMCPMC4636497

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.