ArticleGlycobiology2016
Protein O-linked glycosylation in the plant pathogen Ralstonia solanacearum.
Article in Glycobiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Review
- Sweet complexity:Frontiers in cellular and infection microbiology · 2024Review
- CRISPRi-Mediated Silencing ofJournal of proteome research · 2023Article
- Advances in protein glycosylation and its role in tissue repair and regeneration.Glycoconjugate journal · 2023Review
- Global diversity and distribution of prophages are lineage-specific within the Ralstonia solanacearum species complex.BMC genomics · 2022Article
- Sculpting the BacterialmBio · 2022Article
- Impairment of a cyanobacterial glycosyltransferase that modifies a pilin results in biofilm development.Environmental microbiology reports · 2022Article
- Glycopeptide-Centric Approaches for the Characterization of Microbial Glycoproteomes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Burkholderia PglL enzymes are Serine preferring oligosaccharyltransferases which target conserved proteins across the Burkholderia genus.Communications biology · 2021Article
- Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants.Scientific reports · 2021Article
- Bacterial Glycoengineering as a Biosynthetic Route to Customized Glycomolecules.Advances in biochemical engineering/biotechnology · 2021Article
- Open Database Searching Enables the Identification and Comparison of Bacterial Glycoproteomes without Defining Glycan Compositions Prior to Searching.Molecular & cellular proteomics : MCP · 2020Article
- Virulence of the emerging pathogen,Future microbiology · 2020Article
- Loss ofmSphere · 2019Article
- Expanding our understanding of the role of microbial glycoproteomes through high-throughput mass spectrometry approaches.Glycoconjugate journal · 2019Article
- Type IV Pilin Post-Translational Modifications Modulate Material Properties of Bacterial Colonies.Biophysical journal · 2019Article
- A General ProteinFrontiers in microbiology · 2018Article
- Engineering and Dissecting the Glycosylation Pathway of a Streptococcal Serine-rich Repeat Adhesin.The Journal of biological chemistry · 2016Article
- Development of Rare Bacterial Monosaccharide Analogs for Metabolic Glycan Labeling in Pathogenic Bacteria.ACS chemical biology · 2016Article
- Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis.Current microbiology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Ralstonia solanacearum is one of the most lethal phytopathogens in the world. Due to its broad host range, it can cause wilting disease in many plant species of economic interest. In this work, we identified the O-oligosaccharyltransferase (O-OTase) responsible for protein O-glycosylation in R. solanacearum. An analysis of the glycoproteome revealed that 20 proteins, including type IV pilins are substrates of this general glycosylation system. Although multiple glycan forms were identified, the majority of the glycopeptides were modified with a pentasaccharide composed of HexNAc-(Pen)-dHex(3), similar to the O antigen subunit present in the lipopolysaccharide of multiple R. solanacearum strains. Disruption of the O-OTase led to the total loss of protein glycosylation, together with a defect in biofilm formation and reduced pathogenicity towards tomato plants. Comparative proteomic analysis revealed that the loss of glycosylation is not associated with widespread proteome changes. Only the levels of a single glycoprotein, the type IV pilin, were diminished in the absence of glycosylation. In parallel, disruption of glycosylation triggered an increase in the levels of a surface lectin homologous to Pseudomonas PA-IIL. These results reveal the important role of glycosylation in the pathogenesis of R. solanacearum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.