ArticleScientific reports2015
Human corneal stromal stem cells support limbal epithelial cells cultured on RAFT tissue equivalents.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 72 citations in OpenAlex.
- Limbal Epithelial Stem Cells in Review: Immune and Lymphangiogenic Privilege and Their Clinical Relevance.Cells · 2026Review
- Caveolin 1 and 2 enhance the proliferative capacity of BCAM-positive corneal progenitors.Scientific reports · 2025Article
- Multiple Stem/Progenitor Cells Isolated from the Limbus.Current stem cell research & therapy · 2025Review
- Anti-Inflammatory and Anti-(Lymph)angiogenic Properties of an ABCB5+ Limbal Mesenchymal Stem Cell Population.International journal of molecular sciences · 2024Article
- Extracellular Vesicles Isolated from Equine Adipose-Derived Stromal Stem Cells (ASCs) Mitigate Tunicamycin-Induced ER Stress in Equine Corneal Stromal Stem Cells (CSSCs).Current issues in molecular biology · 2024Article
- Posterior corneoscleral limbus: Architecture, stem cells, and clinical implications.Progress in retinal and eye research · 2023Review
- Recent Approaches to the Modification of Collagen Biomatrix as a Corneal Biomatrix and Its Cellular Interaction.Polymers · 2023Review
- Article
- Epigenetic Regulation of Corneal Epithelial Differentiation by TET2.International journal of molecular sciences · 2023Article
- 3D bioprinting as a prospective therapeutic strategy for corneal limbal epithelial stem cell deficiency.International journal of bioprinting · 2023Article
- Corneal Epithelial Regeneration: Old and New Perspectives.International journal of molecular sciences · 2022Review
- Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiation.Cell reports · 2022Article
- Transcriptomic Profiling of Human Limbus-Derived Stromal/Mesenchymal Stem Cells-Novel Mechanistic Insights into the Pathways Involved in Corneal Wound Healing.International journal of molecular sciences · 2022Article
- Hsa-miR-143-3p inhibits Wnt-β-catenin and MAPK signaling in human corneal epithelial stem cells.Scientific reports · 2022Article
- Regenerative therapy for the Cornea.Progress in retinal and eye research · 2022Review
- High expression of SARS-CoV2 viral entry-related proteins in human limbal stem cells.The ocular surface · 2022Article
- Hsa-miR-150-5p inhibits Wnt-β-catenin signaling in human corneal epithelial stem cells.Molecular vision · 2022Article
- Culture and characterization of canine and feline corneal epithelial organoids: A new tool for the study and treatment of corneal diseases.Frontiers in veterinary science · 2022Article
- Biofabrication of Artificial Stem Cell Niches in the Anterior Ocular Segment.Bioengineering (Basel, Switzerland) · 2021Review
- The Limbal Niche and Regenerative Strategies.Vision (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human limbal epithelial cells (HLE) and corneal stromal stem cells (CSSC) reside in close proximity in vivo in the corneal limbal stem cell niche. However, HLE are typically cultured in vitro without supporting niche cells. Here, we re-create the cell-cell juxtaposition of the native environment in vitro, to provide a tool for investigation of epithelial-stromal cell interactions and to optimize HLE culture conditions for potential therapeutic application. RAFT (Real Architecture For 3D Tissue) tissue equivalents (TEs) were used as a 3-dimensional substrate for co-culturing HLE and CSSC. Our results demonstrate that a monolayer of HLE that maintained expression of p63α, ABCB5, CK8 and CK15 (HLE markers), formed on the surface of RAFT TEs within 13 days of culture. CSSC remained in close proximity to HLE and maintained expression of mesenchymal stem cell markers. This simple technique has a short preparation time of only 15 days with the onset of HLE layering and differentiation observed. Furthermore, co-cultivation of HLE with another niche cell type (CSSC) directly on RAFT TEs, eliminates the requirement for animal-derived feeder cells. RAFT TEs may be useful for future therapeutic delivery of multiple cell types to restore the limbal niche following ocular surface injury or disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.