ArticleScientific reports2015
Physiological Function of Rac Prophage During Biofilm Formation and Regulation of Rac Excision in Escherichia coli K-12.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 41 citations in OpenAlex.
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- Clonal interference and genomic repair during strain coexistence in the gut.PLoS genetics · 2025Article
- Draft genome sequences of a novelMicrobiology resource announcements · 2025Article
- A transcription factor from the cryptic Escherichia coli Rac prophage controls both phage and host operons.Nucleic acids research · 2025Article
- A noncanonical intrinsic terminator in the HicAB toxin-antitoxin operon promotes the transmission of conjugative antibiotic resistance plasmids.Nucleic acids research · 2025Article
- Type I toxin-antitoxin systems in bacteria: from regulation to biological functions.EcoSal Plus · 2024Review
- Clinically relevant mutations in regulatory regions of metabolic genes facilitate early adaptation to ciprofloxacin in Escherichia coli.Nucleic acids research · 2024Article
- Lethal perturbation of an Escherichia coli regulatory network is triggered by a restriction-modification system's regulator and can be mitigated by excision of the cryptic prophage Rac.Nucleic acids research · 2024Article
- A systematic analysis of marine lysogens and proviruses.Nature communications · 2023Article
- The Role of Temperate Phages in Bacterial Pathogenicity.Microorganisms · 2023Review
- Prophage Tracer: precisely tracing prophages in prokaryotic genomes using overlapping split-read alignment.Nucleic acids research · 2021Article
- Activating the Cpx response induces tolerance to antisense PNA delivered by an arginine-rich peptide inMolecular therapy. Nucleic acids · 2021Article
- Xenogeneic silencing relies on temperature-dependent phosphorylation of the host H-NS protein in Shewanella.Nucleic acids research · 2021Article
- Whole-Genome Analysis Reveals That the Nucleoid Protein IHF Predominantly Binds to the Replication OriginFrontiers in microbiology · 2021Article
- Environmental pH is a key modulator of Staphylococcus aureus biofilm development under predation by the virulent phage phiIPLA-RODI.The ISME journal · 2021Article
- Prophage encoding toxin/antitoxin system PfiT/PfiA inhibits Pf4 production in Pseudomonas aeruginosa.Microbial biotechnology · 2020Article
- Cryptic prophages in a blaJournal of microbiology (Seoul, Korea) · 2020Article
- Biofilms: hot spots of horizontal gene transfer (HGT) in aquatic environments, with a focus on a new HGT mechanism.FEMS microbiology ecology · 2020Review
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rac or rac-like prophage harbors many genes with important physiological functions, while it remains excision-proficient in several bacterial strains including Escherichia coli, Salmonella spp. and Shigella spp. Here, we found that rac excision is induced during biofilm formation, and the isogenic stain without rac is more motile and forms more biofilms in nutrient-rich medium at early stages in E. coli K-12. Additionally, the presence of rac genes increases cell lysis during biofilm development. In most E. coli strains, rac is integrated into the ttcA gene which encodes a tRNA-thioltransferase. Rac excision in E. coli K-12 leads to a functional change of TtcA, which results in reduced fitness in the presence of carbenicillin. Additionally, we demonstrate that YdaQ (renamed as XisR) is the excisionase of rac in E. coli K-12, and that rac excision is induced by the stationary sigma factor RpoS through inducing xisR expression. Taken together, our results reveal that upon rac integration, not only are new genes introduced into the host, but also there is a functional change in a host enzyme. Hence, rac excision is tightly regulated by host factors to control its stability in the host genome under different stress conditions.
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