Evidence map›Paper›PMID 26530684›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Expression and Purification of Mitochondrial RNA Polymerase and Transcription Factor A from Drosophila melanogaster.

John P Gajewski, Jamie J Arnold, Tiina S Salminen, Laurie S Kaguni, Craig E Cameron

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 73% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

John P GajewskiDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, 201 Althouse Laboratory, University Park, PA, 16802, USA.
Jamie J ArnoldDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, 201 Althouse Laboratory, University Park, PA, 16802, USA.
Tiina S SalminenInstitute of Biosciences and Medical Technology, University of Tampere, Tampere, Finland.
Laurie S KaguniInstitute of Biosciences and Medical Technology, University of Tampere, Tampere, Finland.
Craig E CameronDepartment of Biochemistry and Molecular Biology, The Pennsylvania State University, 201 Althouse Laboratory, University Park, PA, 16802, USA. cec9@psu.edu.
Pennsylvania State University · USMichigan State University · USTampere University · FI

Funding

Mitochondrial DNA Replisome: Mechanism, Structure and PhysiologyR01GM045295 · NIGMS · MICHIGAN STATE UNIVERSITY · PI KAGUNI, LAURIE SIMON · 1991 to 2013
$4.0M
NIGMS NIH HHS R01 GM045295PHS HHS 45295
6 · The paper itself

Abstract

Mitochondrial gene expression is essential in all organisms. Our understanding of mitochondrial transcription on a biochemical level has been limited by the inability to purify the individual protein components involved in mitochondrial gene expression. Recently, new systems have been identified that permit purification of these proteins from bacteria. However, the generalizability of these systems is not clear. Here, we have applied the technology from the Cameron lab to express and purify mitochondrial RNA polymerase and transcription factor A from Drosophila melanogaster. We show that the use of SUMO system to produce SUMO fusion proteins in bacteria is effective not only for the human and mouse proteins, but also for the fly proteins. The application of this system to produce the mitochondrial proteins from other organisms should permit detailed understanding of mitochondrial transcription from any organism.

Indexed as

AnimalsBacteriaCysteine EndopeptidasesDNA-Directed RNA PolymerasesDrosophila melanogasterDrosophila ProteinsMitochondriaRecombinant Fusion ProteinsRNARNA, MitochondrialTranscription FactorsTranscription, GeneticCysteine EndopeptidasesDNA-Directed RNA PolymerasesDrosophila ProteinsmtRNApol protein, DrosophilaRecombinant Fusion ProteinsRNARNA, MitochondrialTFAM protein, DrosophilaTranscription FactorsUlp1 proteaseMitochondriaMitochondrial RNA polymeraseMitochondrial transcriptionProtein expressionProtein purificationTranscription factor A

Identifiers

PMID26530684
PMCPMC4750393
OpenAlexW2474238639

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.