Evidence map›Paper›PMID 26530671›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Analysis of Translesion DNA Synthesis by the Mitochondrial DNA Polymerase γ.

William C Copeland, Rajesh Kasiviswanathan, Matthew J Longley

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Structure and function relationships in mammalian DNA polymerases.Cellular and molecular life sciences : CMLS · 2020
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

William C CopelandMitochondrial DNA Replication Group, Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T.W. Alexander Dr, Building 101, Rm E316, Research Triangle Park, NC, 27709, USA. copelan1@niehs.nih.gov.
Rajesh KasiviswanathanMitochondrial DNA Replication Group, Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T.W. Alexander Dr, Building 101, Rm E316, Research Triangle Park, NC, 27709, USA.
Matthew J LongleyMitochondrial DNA Replication Group, Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, 111 T.W. Alexander Dr, Building 101, Rm E316, Research Triangle Park, NC, 27709, USA.

Funding

Molecular Characterization Of The Mitochondrial Dna PolymeraseZIAES065078 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI COPELAND, WILLIAM · 2009 to 2025
$25.4M
Human Mitochondrial Dna Polymerase With Anti-hiv NucleotidesZIAES065080 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI COPELAND, WILLIAM · 2009 to 2025
$9.6M
Molecular Study Of The Mitochondrial DNA PolymeraseZ01ES065078 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI COPELAND, WILLIAM · 1994 to 2008
$2.8M
Mitochondrial DNA Polymerase With Anti-HIV NucleotidesZ01ES065080 · NIEHS · NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES · PI COPELAND, WILLIAM · 1995 to 2008
$763k
Intramural NIH HHS Z01 ES065078Intramural NIH HHS Z01 ES065080
6 · The paper itself

Abstract

Mitochondrial DNA is replicated by the nuclear-encoded DNA polymerase γ (pol γ) which is composed of a single 140 kDa catalytic subunit and a dimeric 55 kDa accessory subunit. Mitochondrial DNA is vulnerable to various forms of damage, including several types of oxidative lesions, UV-induced photoproducts, chemical adducts from environmental sources, as well as alkylation and inter-strand cross-links from chemotherapy agents. Although many of these lesions block DNA replication, pol γ can bypass some lesions by nucleotide incorporation opposite a template lesion and further extension of the DNA primer past the lesion. This process of translesion synthesis (TLS) by pol γ can occur in either an error-free or an error-prone manner. Assessment of TLS requires extensive analysis of oligonucleotide substrates and replication products by denaturing polyacrylamide sequencing gels. This chapter presents protocols for the analysis of translesion DNA synthesis.

Indexed as

DNA DamageDNA-Directed DNA PolymeraseDNA, MitochondrialDNA Polymerase gammaDNA RepairDNA ReplicationElectrophoresis, Gel, Two-DimensionalHumansMitochondriaOxidation-ReductionDNA-Directed DNA PolymeraseDNA, MitochondrialDNA Polymerase gammaDNA polymerase γDNA repairDNA replicationEnzyme assaysMitochondrial DNA polymerasePOLGTranslesion synthesis

Identifiers

PMID26530671
PMCPMC5000860

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.