ArticlePlant physiology2016
Constitutive and Companion Cell-Specific Overexpression of AVP1, Encoding a Proton-Pumping Pyrophosphatase, Enhances Biomass Accumulation, Phloem Loading, and Long-Distance Transport.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 67 citations in OpenAlex.
- The Contribution of Phloem Metabolism to Leaf Respiration: New Insights Into an Old Problem.Plant, cell & environment · 2026Article
- Genetically Modified Plants in Agriculture.Biology · 2026Review
- UBIQUITIN-CONJUGATING ENZYME34 mediates pyrophosphatase AVP1 turnover and regulates abiotic stress responses in Arabidopsis.Plant physiology · 2025Article
- Regulated to respond: dual localization and dynamic control of AVP1 in plant carbon partitioning.Frontiers in plant science · 2025Review
- Evaluation of Pyrophosphate-Driven Proton Pumps inMicroorganisms · 2024Article
- Overexpression of vacuolar HFrontiers in plant science · 2024Article
- Exploration of the Catalytic Cycle Dynamics of Vigna Radiata HThe Journal of membrane biology · 2023Article
- Analysis of companion cell and phloem metabolism using a transcriptome-guided model of Arabidopsis metabolism.Plant physiology · 2023Article
- Phosphorylation of SWEET sucrose transporters regulates plant root:shoot ratio under drought.Nature plants · 2022Article
- Proton-pumping pyrophosphatase homeolog expression is a dynamic trait in bread wheat (Plant direct · 2021Article
- The HHorticulture research · 2021Article
- Phenotypic characterization ofApplications in plant sciences · 2020Article
- Improved Yield and Photosynthate Partitioning in AVP1 Expressing Wheat (Frontiers in plant science · 2020Article
- Lack of Vacuolar HFrontiers in plant science · 2020Article
- Biotechnological strategies for improved photosynthesis in a future of elevated atmospheric COPlanta · 2019Review
- A Ma10 gene encoding P-type ATPase is involved in fruit organic acid accumulation in apple.Plant biotechnology journal · 2019Article
- Tissue-specific transcriptomic profiling of Plantago major provides insights for the involvement of vasculature in phosphate deficiency responses.Molecular genetics and genomics : MGG · 2019Article
- The flip side of theThe Journal of biological chemistry · 2019Article
- Utilizing trait networks and structural equation models as tools to interpret multi-trait genome-wide association studies.Plant methods · 2019Article
- Cell Wall Invertase 3 Affects Cassava Productivity via Regulating Sugar Allocation From Source to Sink.Frontiers in plant science · 2019Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant productivity is determined in large part by the partitioning of assimilates between the sites of production and the sites of utilization. Proton-pumping pyrophosphatases (H(+)-PPases) are shown to participate in many energetic plant processes, including general growth and biomass accumulation, CO2 fixation, nutrient acquisition, and stress responses. H(+)-PPases have a well-documented role in hydrolyzing pyrophosphate (PPi) and capturing the released energy to pump H(+) across the tonoplast and endomembranes to create proton motive force (pmf). Recently, an additional role for H(+)-PPases in phloem loading and biomass partitioning was proposed. In companion cells (CCs) of the phloem, H(+)-PPases localize to the plasma membrane rather than endomembranes, and rather than hydrolyzing PPi to create pmf, pmf is utilized to synthesize PPi. Additional PPi in the CCs promotes sucrose oxidation and ATP synthesis, which the plasma membrane P-type ATPase in turn uses to create more pmf for phloem loading of sucrose via sucrose-H(+) symporters. To test this model, transgenic Arabidopsis (Arabidopsis thaliana) plants were generated with constitutive and CC-specific overexpression of AVP1, encoding type 1 ARABIDOPSIS VACUOLAR PYROPHOSPHATASE1. Plants with both constitutive and CC-specific overexpression accumulated more biomass in shoot and root systems. (14)C-labeling experiments showed enhanced photosynthesis, phloem loading, phloem transport, and delivery to sink organs. The results obtained with constitutive and CC-specific promoters were very similar, such that the growth enhancement mediated by AVP1 overexpression can be attributed to its role in phloem CCs. This supports the model for H(+)-PPases functioning as PPi synthases in the phloem by arguing that the increases in biomass observed with AVP1 overexpression stem from improved phloem loading and transport.
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