Evidence map›Paper›PMID 26530282›Full record

ArticleStem cell reviews and reports2016

Primed Mesenchymal Stem Cells Alter and Improve Rat Medial Collateral Ligament Healing.

Erin E Saether, Connie S Chamberlain, Erdem Aktas, Ellen M Leiferman, Stacey L Brickson, Ray Vanderby

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reviews and reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 33 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Exosome Cell Origin AffectsTissue engineering. Part A · 2023
    Article
  6. Review
  7. Review
  8. Article
  9. Review
  10. Tendon and Ligament Healing and Current Approaches to Tendon and Ligament Regeneration.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020
    Review
  11. Article
  12. Article
  13. Review
  14. The therapeutic potential of the mesenchymal stem cell secretome in ischaemic stroke.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2018
    Review
  15. Article
  16. Article
  17. Article
  18. Review
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Erin E SaetherDepartment of Biomedical Engineering, University of Wisconsin, 1111 Highland Ave, WIMR Room 5059, Madison, WI, 53705, USA.
Connie S ChamberlainDepartment of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI, 53705, USA.
Erdem AktasDepartment of Orthopedics, Ankara Oncology Research and Training Hospital, Ankara, Turkey.
Ellen M LeifermanDepartment of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI, 53705, USA.
Stacey L BricksonDepartment of Orthopedics and Rehabilitation, University of Wisconsin, Madison, WI, 53705, USA.
Ray VanderbyDepartment of Biomedical Engineering, University of Wisconsin, 1111 Highland Ave, WIMR Room 5059, Madison, WI, 53705, USA. Vanderby@ortho.wisc.edu.
University of Wisconsin–Madison · USMinistry of Health · TR

Funding

Modulation of the Immune System to Improve Ligament/Ligament Graft HealingR01AR059916 · NIAMS · UNIVERSITY OF WISCONSIN-MADISON · PI MURPHY, WILLIAM L., VANDERBY, RAY · 2010 to 2014
$1.4M
NIAMS NIH HHS AR059916NIAMS NIH HHS R01 AR059916
6 · The paper itself

Abstract

Cell therapy with mesenchymal stem cells (MSCs) can improve tissue healing. It is possible, however, that priming MSCs prior to implantation can further enhance their therapeutic benefit. This study was then performed to test whether priming MSCs to be more anti-inflammatory would enhance healing in a rat ligament model, i.e. a medial collateral ligament (MCL). MSCs were primed for 48 h using polyinosinic acid and polycytidylic acid (Poly (I:C)) at a concentration of 1 μg/ml. Rat MCLs were surgically transected and administered 1 × 10(6) cells in a carrier solution at the time of injury. A series of healing metrics were analyzed at days 4 and 14 post-injury in the ligaments that received primed MSCs, unprimed MSCs, or no cells (controls). Applying primed MSCs beneficially altered healing by affecting endothelialization, type 2 macrophage presence, apoptosis, procollagen 1α, and IL-1Ra levels. When analyzing MSC localization, both primed and unprimed MSCs co-localized with endothelial cells and pericytes suggesting a supportive role in angiogenesis. Priming MSCs prior to implantation altered key ligament healing events, resulted in a more anti-inflammatory environment, and improved healing.

Indexed as

Mesenchymal Stem Cell TransplantationNeovascularization, PhysiologicAnimalsApoptosisCell MovementCollagen Type ICollagen Type I, alpha 1 ChainCollateral LigamentsEndothelial CellsHumansInterleukin 1 Receptor Antagonist ProteinMacrophagesMaleMesenchymal Stem CellsPericytesPoly I-CCollagen Type ICollagen Type I, alpha 1 ChainInterleukin 1 Receptor Antagonist ProteinPoly I-CAngiogenesisInflammationLigamentMacrophageMesenchymal stem cellsPolycytidylic acid

Identifiers

PMID26530282
PMCPMC4968884
OpenAlexW2279938184

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.