Evidence map›Paper›PMID 26530043›Full record

ArticleJournal of cellular physiology2016

Complex-I Alteration and Enhanced Mitochondrial Fusion Are Associated With Prostate Cancer Progression.

Julie V Philley, Anbarasu Kannan, Wenyi Qin, Edward R Sauter, Mitsuo Ikebe, Kate L Hertweck, Dean A Troyer, Oliver J Semmes, Santanu Dasgupta

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 56 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Julie V PhilleyDepartment of Medicine, The University of Texas Health Science Center at Tyler, Tyler, Texas.
Anbarasu KannanDepartment of Cellular and Molecular Biology, The University of Texas Health Science Center at Tyler, Tyler, Texas.
Wenyi QinDepartment of Surgery, The University of Texas Health Science Center at Tyler, Tyler, Texas.
Edward R SauterDepartment of Surgery, The University of Texas Health Science Center at Tyler, Tyler, Texas.
Mitsuo IkebeDepartment of Cellular and Molecular Biology, The University of Texas Health Science Center at Tyler, Tyler, Texas.
Kate L HertweckDepartment of Biology, The University of Texas at Tyler, Tyler, Texas.
Dean A TroyerDepartment of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, Virginia.
Oliver J SemmesDepartment of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, Virginia.
Santanu DasguptaDepartment of Cellular and Molecular Biology, The University of Texas Health Science Center at Tyler, Tyler, Texas.
The University of Texas Health Science Center at Tyler · USEastern Virginia Medical School · USThe University of Texas at Tyler · US

Funding

Regulation of Myosin Phosphorylation in Smooth MuscleR01HL073050 · NHLBI · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI IKEBE, MITSUO · 2003 to 2013
$4.0M
Rac1 in Smooth MuscleR01HL111696 · NHLBI · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI IKEBE, MITSUO · 2012 to 2016
$1.7M
NHLBI NIH HHS R01 HL073050NHLBI NIH HHS R01 HL111696
6 · The paper itself

Abstract

Mitochondria (mt) encoded respiratory complex-I (RCI) mutations and their pathogenicity remain largely unknown in prostate cancer (PCa). Little is known about the role of mtDNA loss on mt integrity in PCa. We determined mtDNA mutation in human and mice PCa and assessed the impact of mtDNA depletion on mt integrity. We also examined whether the circulating exosomes from PCa patients are transported to mt and carry mtDNA or mt proteins. We have employed next generation sequencing of the whole mt genome in human and Hi-myc PCa. The impact of mtDNA depletion on mt integrity, presence of mtDNA, and protein in sera exosomes was determined. A co-culture of human PCa cells and the circulating exosomes followed by confocal imaging determined co-localization of exosomes and mt. We observed frequent RCI mutations in human and Hi-myc PCa which disrupted corresponding complex protein expression. Depletion of mtDNA in PCa cells influenced mt integrity, increased expression of MFN1, MFN2, PINK1, and decreased expression of MT-TFA. Increased mt fusion and expression of PINK1 and DNM1L were also evident in the Hi-myc tumors. RCI-mtDNA, MFN2, and IMMT proteins were detected in the circulating exosomes of men with benign prostate hyperplasia (BPH) and progressive PCa. Circulating exosomes and mt co-localized in PCa cells. Our study identified new pathogenic RCI mutations in PCa and defined the impact of mtDNA loss on mt integrity. Presence of mtDNA and mt proteins in the circulating exosomes implicated their usefulness for biomarker development.

Indexed as

Mitochondrial DynamicsAnimalsBiomarkers, TumorCell Line, TumorCoculture TechniquesDisease ProgressionDNA, MitochondrialElectron Transport Complex IExosomesGene Expression Regulation, NeoplasticGenes, mycGenetic Association StudiesGenetic Predisposition to DiseaseHaplotypesHumansMaleBiomarkers, TumorDNA, MitochondrialElectron Transport Complex I

Identifiers

PMID26530043
PMCPMC5741292
OpenAlexW2169890296

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.