ArticleScientific reports2015
Compartmentalized microchannel array for high-throughput analysis of single cell polarized growth and dynamics.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Recent Advances in Microfluidics for the Early Detection of Plant Diseases in Vegetables, Fruits, and Grains Caused by Bacteria, Fungi, and Viruses.Journal of agricultural and food chemistry · 2024Review
- The clock in growing hyphae and their synchronization in Neurospora crassa.Communications biology · 2024Article
- A microfluidic device for simultaneous detection of enzyme secretion and elongation of a single hypha.Frontiers in microbiology · 2023Article
- Bending stiffness ofLab on a chip · 2022Article
- Recent advances of integrated microfluidic suspension cell culture system.Engineering biology · 2021Review
- Platforms for High-Throughput Screening and Force Measurements on Fungi and Oomycetes.Micromachines · 2021Review
- A versatile microfluidic platform measures hyphal interactions between Fusarium graminearum and Clonostachys rosea in real-time.Communications biology · 2021Article
- Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow.Micromachines · 2020Article
- A 3D Printed Device for Easy and Reliable Quantification of Fungal Chemotropic Growth.Frontiers in microbiology · 2020Article
- Intracellular mechanisms of fungal space searching in microenvironments.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Increasing access to microfluidics for studying fungi and other branched biological structures.Fungal biology and biotechnology · 2019Article
- Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms.Biomicrofluidics · 2017Article
- Recent Advances in the Analysis of Single Cells.Analytical chemistry · 2017Review
- Synchronizing stochastic circadian oscillators in single cells of Neurospora crassa.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interrogating polarized growth is technologically challenging due to extensive cellular branching and uncontrollable environmental conditions in conventional assays. Here we present a robust and high-performance microfluidic system that enables observations of polarized growth with enhanced temporal and spatial control over prolonged periods. The system has built-in tunability and versatility to accommodate a variety of scientific applications requiring precisely controlled environments. Using the model filamentous fungus, Neurospora crassa, our microfluidic system enabled direct visualization and analysis of cellular heterogeneity in a clonal fungal cell population, nuclear distribution and dynamics at the subhyphal level, and quantitative dynamics of gene expression with single hyphal compartment resolution in response to carbon source starvation and exchange. Although the microfluidic device is demonstrated on filamentous fungi, the technology is immediately extensible to a wide array of other biosystems that exhibit similar polarized cell growth, with applications ranging from bioenergy production to human health.
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Registered trials
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